Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2002 Mar;128(3):876-84.
doi: 10.1104/pp.010843.

Evidence supporting a role of jasmonic acid in Arabidopsis leaf senescence

Yuehui He  1 , Hirotada Fukushige, David F Hildebrand, Susheng Gan
Affiliations
Comparative Study

Evidence supporting a role of jasmonic acid in Arabidopsis leaf senescence

Yuehui He et al. Plant Physiol. 2002 Mar.

Abstract

In this work, the role of jasmonic acid (JA) in leaf senescence is examined. Exogenous application of JA caused premature senescence in attached and detached leaves in wild-type Arabidopsis but failed to induce precocious senescence of JA-insensitive mutant coi1 plants, suggesting that the JA-signaling pathway is required for JA to promote leaf senescence. JA levels in senescing leaves are 4-fold higher than in non-senescing ones. Concurrent with the increase in JA level in senescing leaves, genes encoding the enzymes that catalyze most of the reactions of the JA biosynthetic pathway are differentially activated during leaf senescence in Arabidopsis, except for allene oxide synthase, which is constitutively and highly expressed throughout leaf development. Arabidopsis lipoxygenase 1 (cytoplasmic) expression is greatly increased but lipoxygenase 2 (plastidial) expression is sharply reduced during leaf senescence. Similarly, AOC1 (allene oxide cyclase 1), AOC2, and AOC3 are all up-regulated, whereas AOC4 is down-regulated with the progression of leaf senescence. The transcript levels of 12-oxo-PDA reductase 1 and 12-oxo-PDA reductase 3 also increase in senescing leaves, as does PED1 (encoding a 3-keto-acyl-thiolase for beta-oxidation). This represents the first report, to our knowledge, of an increase in JA levels and expression of oxylipin genes during leaf senescence, and indicates that JA may play a role in the senescence program.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Biosynthetic pathway of JA. 13(S)-HPOT, (9Z,11E,15Z,13S)- 13-hydroperoxy-9,11,15-octadecatrienoic acid; 12-oxo-PDA, 12-oxo-10,15(Z)-octadecatrienoic acid; PED1, peroxisome defective 1, a 3-keto-acyl-thiolase; PKT1/2, 3-keto-acyl-thiolase 1 and 2; OPC-8:0, 3-oxo-2(2′(Z)-pentenyl)-cyclopentane-1-octanoic acid.
Figure 2
Figure 2
Promotion of leaf senescence in Arabidopsis by JA. Wild-type Col-gl1 (A and B) and JA-insensitive mutant coi1 plants (C) were grown on phytoagar medium containing 0 (A) or 30 (B and C) μm JA for 12 d. D, Detached young Col-gl1 leaves treated with water or 30 μm JA for 4 d under darkness. E, Variable fluorescence (Fv)/maximal fluorescence (Fm) values of leaves shown in D. F, Expression of the senescence-specific marker gene SAG12 in leaves shown in D.
Figure 3
Figure 3
RT-PCR analysis of the expression of JA-responsive marker gene PDF1.2 during leaf senescence. EL, About 50% of the fully expanded leaves; NS, fully expanded, NS leaves; ES, up to 25% of a leaf shows yellowing; LS, more than 50% of a leaf shows yellowing.
Figure 4
Figure 4
Expression of LOXs during leaf senescence in Arabidopsis. A, RNA gel-blot analysis of LOX1 and LOX2. B, RT-PCR analysis of LOX3 and LOX4. EL, NS, ES, and LS are as described in legend to Figure 3.
Figure 5
Figure 5
Constitutive expression of AOS throughout leaf development. EL, NS, ES, and LS are as described in legend to Figure 3.
Figure 6
Figure 6
RT-PCR analysis of the expression of AOCs during leaf senescence. EL, NS, ES, and LS are as described in legend to Figure 3.
Figure 7
Figure 7
RT-PCR analysis of the expression of OPRs during leaf senescence. EL, NS, ES, and LS are as described in legend to Figure 3.
Figure 8
Figure 8
RNA gel-blot analysis of thiolase genes during leaf senescence. EL, NS, ES, and LS are as described in legend to Figure 3.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Albrecht T, Kehlen A, Stahl K, Knöfel H-D, Sembdner G, Weiler EW. Quantification of rapid, transient increases in jasmonic acid in wounded plants using a monoclonal antibody. Planta. 1993;191:86–94.
    1. Bell E, Mullet JE. Characterization of an Arabidopsis lipoxygenase gene responsive to methyl jasmonate and wounding. Plant Physiol. 1993;103:1133–1137. - PMC - PubMed
    1. Biesgen C, Weiler EW. Structure and regulation of OPR1 and OPR2, two closely related genes encoding 12-oxophytodienoic acid-10,11-reductases from Arabidopsis thaliana. Planta. 1999;208:155–165. - PubMed
    1. Bleecker AB, Patterson SE. Last exit: senescence, abscission, and meristem arrest in Arabidopsis. Plant Cell. 1997;9:1169–1179. - PMC - PubMed
    1. Buchanan-Wollaston V. The molecular biology of leaf senescence. J Exp Bot. 1997;48:181–199.

Publication types

MeSH terms