Human fragile site FRA16B DNA excludes nucleosomes in the presence of distamycin
- PMID: 11880377
- DOI: 10.1074/jbc.M200901200
Human fragile site FRA16B DNA excludes nucleosomes in the presence of distamycin
Abstract
Human fragile sites are weak staining gaps in chromosomes generated by specific culture conditions. The short CGG repeating DNA derived from folate-sensitive fragile sites has been shown to exclude single nucleosomes. To test whether this nucleosome exclusion model provides a general molecular mechanism for the formation of fragile sites, a different class of fragile site, the 33-base pair AT-rich repeating DNAs derived from the rare distamycin-inducible site, FRA16B, was examined for its ability to assemble single nucleosomes and nucleosome arrays using in vitro nucleosome reconstitution methods. The FRA16B DNA fragments strongly exclude nucleosome assembly only in the presence of distamycin, and increasing the number of 33-bp repeats increases the effect of distamycin in the destabilization of the nucleosome formation, suggesting a common mechanism for the formation of fragile sites.
Similar articles
-
Human chromosome fragility.Biochim Biophys Acta. 2008 Jan;1779(1):3-16. doi: 10.1016/j.bbagrm.2007.10.005. Epub 2007 Dec 3. Biochim Biophys Acta. 2008. PMID: 18078840 Review.
-
Long CCG triplet repeat blocks exclude nucleosomes: a possible mechanism for the nature of fragile sites in chromosomes.J Mol Biol. 1996 Nov 8;263(4):511-6. doi: 10.1006/jmbi.1996.0593. J Mol Biol. 1996. PMID: 8918933
-
Human chromosomal fragile site FRA16B is an amplified AT-rich minisatellite repeat.Cell. 1997 Feb 7;88(3):367-74. doi: 10.1016/s0092-8674(00)81875-9. Cell. 1997. PMID: 9039263
-
Induction of distamycin A-inducible rare fragile sites and increased sister chromatid exchanges at the fragile site.Hum Genet. 1991 Jul;87(3):254-60. doi: 10.1007/BF00200900. Hum Genet. 1991. PMID: 1864598
-
The rare human fragile site 16B.Cytogenet Genome Res. 2003;100(1-4):85-8. doi: 10.1159/000072841. Cytogenet Genome Res. 2003. PMID: 14526167 Review.
Cited by
-
Chromatin changes in the development and pathology of the Fragile X-associated disorders and Friedreich ataxia.Biochim Biophys Acta. 2012 Jul;1819(7):802-10. doi: 10.1016/j.bbagrm.2011.12.009. Epub 2012 Jan 5. Biochim Biophys Acta. 2012. PMID: 22245581 Free PMC article. Review.
-
Secondary structure formation and DNA instability at fragile site FRA16B.Nucleic Acids Res. 2010 May;38(9):2865-77. doi: 10.1093/nar/gkp1245. Epub 2010 Jan 13. Nucleic Acids Res. 2010. PMID: 20071743 Free PMC article.
-
HIV chromatin is a preferred target for drugs that bind in the DNA minor groove.PLoS One. 2019 Dec 30;14(12):e0216515. doi: 10.1371/journal.pone.0216515. eCollection 2019. PLoS One. 2019. PMID: 31887110 Free PMC article.
-
Fragile sites, chromosomal lesions, tandem repeats, and disease.Front Genet. 2022 Nov 17;13:985975. doi: 10.3389/fgene.2022.985975. eCollection 2022. Front Genet. 2022. PMID: 36468036 Free PMC article. Review.
-
Pooled analysis of loss of heterozygosity in breast cancer: a genome scan provides comparative evidence for multiple tumor suppressors and identifies novel candidate regions.Am J Hum Genet. 2003 Oct;73(4):748-67. doi: 10.1086/378522. Epub 2003 Sep 16. Am J Hum Genet. 2003. PMID: 13680524 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
