doi: 10.1126/science.1068228.
Epub 2002 Jan 3.
Production of alpha-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning
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- PMID: 11778012
- DOI: 10.1126/science.1068228
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Production of alpha-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning
Science.
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Abstract
The presence of galactose alpha-1,3-galactose residues on the surface of pig cells is a major obstacle to successful xenotransplantation. Here, we report the production of four live pigs in which one allele of the alpha-1,3-galactosyltransferase locus has been knocked out. These pigs were produced by nuclear transfer technology; clonal fetal fibroblast cell lines were used as nuclear donors for embryos reconstructed with enucleated pig oocytes.
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