Gene profiling reveals unknown enhancing and suppressive actions of glucocorticoids on immune cells
- PMID: 11772937
- DOI: 10.1096/fj.01-0245com
Gene profiling reveals unknown enhancing and suppressive actions of glucocorticoids on immune cells
Abstract
Glucocorticoids continue to be the major immunomodulatory agents used in clinical medicine today. However, their actions as anti-inflammatory and immunosuppressive drugs are both beneficial and deleterious. We analyzed the effect of glucocorticoids on the gene expression profile of peripheral blood mononuclear cells from healthy donors. DNA microarray analysis combined with quantitative TaqMan PCR and flow cytometry revealed that glucocorticoids induced the expression of chemokine, cytokine, and complement family members as well as of newly discovered innate immune-related genes, including scavenger and Toll-like receptors. In contrast, glucocorticoids repressed the expression of adaptive immune-related genes. Simultaneous inhibitory and stimulatory effects of glucocorticoids were found on inflammatory T helper subsets and apoptosis-related gene clusters. In cells activated by T cell receptor cross-linking, glucocorticoids down-regulated the expression of specific genes that were previously up-regulated in resting cells, suggesting a potential new mechanism by which they exert positive and negative effects. Considering the broad and continuously renewed interest in glucocorticoid therapy, the profiles we describe here will be useful in designing more specific and efficient treatment strategies.
Similar articles
-
The effects of dexamethasone and dehydroepiandrosterone (DHEA) on cytokines and receptor expression in a human osteoblastic cell line: potential steroid-sparing role for DHEA.Cytokine. 2006 Oct;36(1-2):57-68. doi: 10.1016/j.cyto.2006.10.012. Epub 2006 Dec 11. Cytokine. 2006. PMID: 17161616
-
Global gene profiling reveals novel glucocorticoid induced changes in gene expression of human lens epithelial cells.Mol Vis. 2005 Nov 23;11:1018-40. Mol Vis. 2005. PMID: 16319822
-
cDNA microarray analysis of gene expression changes induced by dexamethasone in cultured human trabecular meshwork cells.Invest Ophthalmol Vis Sci. 2002 Dec;43(12):3691-7. Invest Ophthalmol Vis Sci. 2002. PMID: 12454038
-
A gradient of glucocorticoid sensitivity among helper T cell cytokines.Cytokine Growth Factor Rev. 2016 Oct;31:27-35. doi: 10.1016/j.cytogfr.2016.05.002. Epub 2016 May 13. Cytokine Growth Factor Rev. 2016. PMID: 27235091 Free PMC article. Review.
-
Regulation of cytokine and cytokine receptor expression by glucocorticoids.J Leukoc Biol. 1996 Nov;60(5):563-72. doi: 10.1002/jlb.60.5.563. J Leukoc Biol. 1996. PMID: 8929546 Review.
Cited by
-
Pilot investigations into the mechanistic basis for adverse effects of glucocorticoids in dysferlinopathy.Skelet Muscle. 2024 Aug 9;14(1):19. doi: 10.1186/s13395-024-00350-6. Skelet Muscle. 2024. PMID: 39123261 Free PMC article.
-
Investigating Whether a Combination of Electro-Encephalography and Gene Expression Profiling Can Predict the Risk of Chronic Pain: A Protocol for an Observational Prospective Cohort Study.Brain Sci. 2024 Jun 26;14(7):641. doi: 10.3390/brainsci14070641. Brain Sci. 2024. PMID: 39061381 Free PMC article.
-
Diagnosis and management of non-CAH 46,XX disorders/differences in sex development.Front Endocrinol (Lausanne). 2024 May 15;15:1354759. doi: 10.3389/fendo.2024.1354759. eCollection 2024. Front Endocrinol (Lausanne). 2024. PMID: 38812815 Free PMC article. Review.
-
Glucocorticoid therapy for sepsis in the AI era: a survey on current and future approaches.Comput Struct Biotechnol J. 2024 Apr 12;24:292-305. doi: 10.1016/j.csbj.2024.04.020. eCollection 2024 Dec. Comput Struct Biotechnol J. 2024. PMID: 38681133 Free PMC article. Review.
-
Exploring the complex interplay: gut microbiome, stress, and leptospirosis.Front Microbiol. 2024 Feb 27;15:1345684. doi: 10.3389/fmicb.2024.1345684. eCollection 2024. Front Microbiol. 2024. PMID: 38476949 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical