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. 2001 Oct;8(4):829-39.
doi: 10.1016/s1097-2765(01)00372-0.

The crystal structure of the PX domain from p40(phox) bound to phosphatidylinositol 3-phosphate

J Bravo  1 , D Karathanassis, C M Pacold, M E Pacold, C D Ellson, K E Anderson, P J Butler, I Lavenir, O Perisic, P T Hawkins, L Stephens, R L Williams
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Free article

The crystal structure of the PX domain from p40(phox) bound to phosphatidylinositol 3-phosphate

J Bravo et al. Mol Cell. 2001 Oct.
Free article

Abstract

More than 50 human proteins with a wide range of functions have a 120 residue phosphoinositide binding module known as the PX domain. The 1.7 A X-ray crystal structure of the PX domain from the p40(phox) subunit of NADPH oxidase bound to PtdIns(3)P shows that the PX domain embraces the 3-phosphate on one side of a water-filled, positively charged pocket and reveals how 3-phosphoinositide specificity is achieved. A chronic granulomatous disease (CGD)-associated mutation in the p47(phox) PX domain that abrogates PtdIns(3)P binding maps to a conserved Arg that does not directly interact with the phosphoinositide but instead appears to stabilize a critical lipid binding loop. The SH3 domain present in the full-length protein does not affect soluble PtdIns(3)P binding to the p40(phox) PX domain.

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