Review
doi: 10.1107/s0907444901010824.
Epub 2001 Sep 21.
Solution solution: using NMR models for molecular replacement
Affiliations
- PMID: 11567160
- DOI: 10.1107/s0907444901010824
Item in Clipboard
Review
Solution solution: using NMR models for molecular replacement
Acta Crystallogr D Biol Crystallogr.
2001 Oct.
Abstract
NMR structures can serve as a good source of search models in crystal structure determination by molecular replacement. However, owing to the inherent problems of NMR models, this procedure is not always straightforward. Here, an updated overview is presented with particular emphasis on the preparation of NMR search models and the latest trends in methodology. An experimental protocol developed recently is described and results on its use in solving a new structure as well as its test against a difficult published case are presented.
Similar articles
-
Ribosomal protein L9: a structure determination by the combined use of X-ray crystallography and NMR spectroscopy.J Mol Biol. 1996 Dec 20;264(5):1058-71. doi: 10.1006/jmbi.1996.0696. J Mol Biol. 1996. PMID: 9000630
-
Macromolecule structure determination using NMR data and molecular simulation techniques.Am Biotechnol Lab. 1990 Jun;8(8):18-24. Am Biotechnol Lab. 1990. PMID: 1366598
-
Human interleukin-4 and variant R88Q: phasing X-ray diffraction data by molecular replacement using X-ray and nuclear magnetic resonance models.J Mol Biol. 1995 Mar 24;247(2):360-72. doi: 10.1006/jmbi.1994.0144. J Mol Biol. 1995. PMID: 7707380
-
Rosetta Structure Prediction as a Tool for Solving Difficult Molecular Replacement Problems.Methods Mol Biol. 2017;1607:455-466. doi: 10.1007/978-1-4939-7000-1_19. Methods Mol Biol. 2017. PMID: 28573585 Review.
-
Structure determination of α-helical membrane proteins by solution-state NMR: emphasis on retinal proteins.Biochim Biophys Acta. 2014 May;1837(5):578-88. doi: 10.1016/j.bbabio.2013.06.009. Epub 2013 Jul 2. Biochim Biophys Acta. 2014. PMID: 23831435 Review.
Cited by
-
Expression, purification, crystallization and preliminary X-ray crystallographic analysis of a resuscitation-promoting factor from Mycobacterium tuberculosis.Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Oct 1;63(Pt 10):870-3. doi: 10.1107/S1744309107043217. Epub 2007 Sep 19. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007. PMID: 17909292 Free PMC article.
-
The JCSG MR pipeline: optimized alignments, multiple models and parallel searches.Acta Crystallogr D Biol Crystallogr. 2008 Jan;64(Pt 1):133-40. doi: 10.1107/S0907444907050111. Epub 2007 Dec 5. Acta Crystallogr D Biol Crystallogr. 2008. PMID: 18094477 Free PMC article.
-
A benchmark driven guide to binding site comparison: An exhaustive evaluation using tailor-made data sets (ProSPECCTs).PLoS Comput Biol. 2018 Nov 8;14(11):e1006483. doi: 10.1371/journal.pcbi.1006483. eCollection 2018 Nov. PLoS Comput Biol. 2018. PMID: 30408032 Free PMC article.
-
Improved technologies now routinely provide protein NMR structures useful for molecular replacement.Structure. 2011 Jun 8;19(6):757-66. doi: 10.1016/j.str.2011.04.005. Structure. 2011. PMID: 21645849 Free PMC article.
-
Crystal structure of a human MUC16 SEA domain reveals insight into the nature of the CA125 tumor marker.Proteins. 2022 May;90(5):1210-1218. doi: 10.1002/prot.26303. Epub 2022 Jan 25. Proteins. 2022. PMID: 35037700 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
