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. 2001 Jul;36(7):683-7.
doi: 10.1007/s11745-001-0773-x.

Regulation of intestinal apolipoprotein A-I synthesis by dietary phosphatidylcholine in newborn swine

H Wang  1 , J Du, S Lu, Y Yao, F Hunter, D D Black
Affiliations

Regulation of intestinal apolipoprotein A-I synthesis by dietary phosphatidylcholine in newborn swine

H Wang et al. Lipids. 2001 Jul.

Abstract

Phospholipid (PL) from both dietary sources and biliary secretions may be important in the regulation of intestinal apolipoprotein (apo) synthesis. We previously demonstrated the up-regulation of apo A-I secretion by phosphatidylcholine (PC) in a newborn piglet intestinal epithelial cell line. We hypothesized that dietary PC increases small intestinal apo A-I synthesis in vivo in the newborn piglet. Two-day-old female swine were fed by gavage for 48 h. Diets consisted of a formula containing 51% of calories as triacylglycerol providing 180 kcal/kg/24 h. The experimental group (+PC, n = 7) received 1 g/L added soybean PC, and the control group (-PC, n = 7) received no added PC. At the end of the study period, jejunal apo A-I, B, and A-IV synthesis was measured, and apo A-I mRNA levels were quantitated. Jejunal mucosal PL content and serum lipids and apo B and A-I levels were measured. Jejunal apo A-I synthesis was almost twice as high in the +PC group as compared to the -PC group with no difference in apo A-I mRNA levels. Jejunal content of PL was higher in the +PC group than in the -PC group. There were no differences in jejunal apo B and A-IV synthesis or serum levels of lipids and apo-lipoproteins between the two groups. Dietary PC supplementation in newborn swine up-regulated jejunal apo A-I synthesis. Apo A-IV synthesis, which is sensitive to fatty acid flux, was not significantly increased, which suggests a specific effect of PC on apo A-I synthesis. Lumenal PC may be important in the regulation of intestinal apo A-I synthesis in the neonate.

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References

    1. Am J Physiol. 1985 Nov;249(5 Pt 1):G642-8 - PubMed
    1. J Lipid Res. 1986 Jan;27(1):30-9 - PubMed
    1. Lipids. 1973 May;8(5):249-55 - PubMed
    1. Gene. 1993 Jan 30;123(2):173-9 - PubMed
    1. J Biol Chem. 1959 Mar;234(3):466-8 - PubMed

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