Exploring protein interactions by interaction-induced folding of proteins from complementary peptide fragments
- PMID: 11495741
- DOI: 10.1016/s0959-440x(00)00235-9
Exploring protein interactions by interaction-induced folding of proteins from complementary peptide fragments
Abstract
The study of protein--protein interactions is central to understanding the chemical machinery that makes up the living cell. Until recently, facile methods to study these processes in intact, living cells have not existed. Furthermore, the assignment of function to novel proteins relies on demonstrating interactions of these proteins with proteins of known function. This review describes an experimental strategy, devised to study protein--protein interactions in any intact living cells based on protein-fragment complementation assays. Applications to quantitative analysis of interactions, allosteric processes and cDNA library screening are discussed. Recently, the feasibility of employing this strategy in genome-wide biochemical pathway mapping efforts has been demonstrated.
Similar articles
-
Detection of protein-protein interactions by protein fragment complementation strategies.Methods Enzymol. 2000;328:208-30. doi: 10.1016/s0076-6879(00)28399-7. Methods Enzymol. 2000. PMID: 11075347 No abstract available.
-
Finding Cinderella's slipper--proteins that fit.Nat Biotechnol. 1999 Jul;17(7):639-40. doi: 10.1038/10850. Nat Biotechnol. 1999. PMID: 10409352 No abstract available.
-
Beta-lactamase protein fragment complementation assays as in vivo and in vitro sensors of protein protein interactions.Nat Biotechnol. 2002 Jun;20(6):619-22. doi: 10.1038/nbt0602-619. Nat Biotechnol. 2002. PMID: 12042868
-
Mapping biochemical networks with protein-fragment complementation assays.Methods Mol Biol. 2004;261:411-26. doi: 10.1385/1-59259-762-9:411. Methods Mol Biol. 2004. PMID: 15064473 Review.
-
Universal strategies in research and drug discovery based on protein-fragment complementation assays.Nat Rev Drug Discov. 2007 Jul;6(7):569-82. doi: 10.1038/nrd2311. Nat Rev Drug Discov. 2007. PMID: 17599086 Review.
Cited by
-
Ypt1 and TRAPP interactions: optimization of multicolor bimolecular fluorescence complementation in yeast.Methods Mol Biol. 2015;1298:107-16. doi: 10.1007/978-1-4939-2569-8_9. Methods Mol Biol. 2015. PMID: 25800836 Free PMC article.
-
Selecting open reading frames from DNA.Genome Res. 2003 May;13(5):980-90. doi: 10.1101/gr.861503. Genome Res. 2003. PMID: 12727911 Free PMC article.
-
AKT1, LKB1, and YAP1 Revealed as MYC Interactors with NanoLuc-Based Protein-Fragment Complementation Assay.Mol Pharmacol. 2017 Apr;91(4):339-347. doi: 10.1124/mol.116.107623. Epub 2017 Jan 13. Mol Pharmacol. 2017. PMID: 28087810 Free PMC article.
-
Identification of a Fragmented Small GTPase Capable of Conditional Effector Binding.RSC Adv. 2017;7(20):12265-12268. doi: 10.1039/C6RA25575B. Epub 2017 Feb 22. RSC Adv. 2017. PMID: 28966788 Free PMC article.
-
Molecular imaging of drug-modulated protein-protein interactions in living subjects.Cancer Res. 2004 Mar 15;64(6):2113-9. doi: 10.1158/0008-5472.can-03-2972. Cancer Res. 2004. PMID: 15026351 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
