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. 2001 Jun;22(3):321-7.
doi: 10.1023/a:1011118424474.

Identification and characterization of the UL24 gene product of herpes simplex virus type 2

Z Hong-Yan  1 , T Murata, F Goshima, H Takakuwa, T Koshizuka, Y Yamauchi, Y Nishiyama
Affiliations

Identification and characterization of the UL24 gene product of herpes simplex virus type 2

Z Hong-Yan et al. Virus Genes. 2001 Jun.

Abstract

The UL24 gene of herpes simplex virus type 2 (HSV-2) is predicted to encode a 281 amino acid protein with a molecular mass of 30.5 kDa. In this study, the HSV-2 UL24 gene product has been identified by using a rabbit polyclonal antiserum produced against a recombinant protein containing the full-length UL24 gene product of HSV-2 fused to glutathione-S-transferase. The antiserum reacted specifically with a 32 kDa protein in HSV-2 186-infected Vero cells and with 31 and 32 kDa proteins in UL24-expressing Cos-7 cells. Accumulation of UL24 protein to detectable levels required viral DNA synthesis, indicating that the protein was regulated as a late gene. UL24 protein was found to be associated with purified HSV-2 virions and C capsids. Indirect immunofluorescence analysis demonstrated that the UL24-specific fluorescence was detected in perinuclear regions of the cytoplasm and/or in the nucleus as small discrete granules from 9h post infection (hpi). Furthermore, the UL24 protein expressed singly was detected predominantly in the nucleus and slightly in the cytoplasm at 24 h after transfection, with branch-like cytoplasmic protruding structures. Strong nucleolus staining was visible in partial cells.

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References

    1. Virus Res. 1991 Mar;18(2-3):135-50 - PubMed
    1. J Gen Virol. 2000 Oct;81(Pt 10):2397-405 - PubMed
    1. J Gen Virol. 1982 Dec;63(2):277-95 - PubMed
    1. Virology. 1997 Aug 18;235(1):82-93 - PubMed
    1. J Virol. 1998 Mar;72(3):2010-21 - PubMed

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