Anionic phospholipids are involved in membrane targeting of PI 3-kinase gamma
- PMID: 11401516
- DOI: 10.1006/bbrc.2001.4623
Anionic phospholipids are involved in membrane targeting of PI 3-kinase gamma
Abstract
Phosphoinositide 3-kinases (PI 3-kinases) have critical roles in diverse cellular signaling processes and in protein trafficking. In contrast to the class I PI 3-kinases alpha, beta, and delta which bind via src homology 2 (SH2) domains of adaptor proteins to tyrosine kinase receptors, the mechanism of recruitment of the PI 3-kinase gamma to membranes is unknown. We report in vitro experiments using immobilized proteins and small unilamellar vesicles which suggest an involvement of anionic phospholipids in membrane association of PI 3-kinase gamma. Furthermore we provide evidence that the enzyme displays beside the catalytic center a phospholipid binding domain which is essential for enzyme activity.
Copyright 2001 Academic Press.
Similar articles
-
Regulation of membrane traffic by phosphoinositide 3-kinases.J Cell Sci. 2006 Feb 15;119(Pt 4):605-14. doi: 10.1242/jcs.02855. J Cell Sci. 2006. PMID: 16467569 Review.
-
Regulation of class IA PI3Ks.Biochem Soc Trans. 2007 Apr;35(Pt 2):242-4. doi: 10.1042/BST0350242. Biochem Soc Trans. 2007. PMID: 17371249
-
Caveolin scaffolding region and the membrane binding region of SRC form lateral membrane domains.Biochemistry. 2003 Jan 14;42(1):42-56. doi: 10.1021/bi012097n. Biochemistry. 2003. PMID: 12515538
-
Probing phosphoinositide functions in signaling and membrane trafficking.Trends Cell Biol. 2005 May;15(5):259-68. doi: 10.1016/j.tcb.2005.03.008. Trends Cell Biol. 2005. PMID: 15866030 Review.
-
A new mechanism for the regulation of Gab1 recruitment to the plasma membrane.J Cell Sci. 2009 Jan 1;122(Pt 1):55-64. doi: 10.1242/jcs.037226. Epub 2008 Dec 2. J Cell Sci. 2009. PMID: 19050043
Cited by
-
Phosphoinositide 3-kinase gamma has multiple phospholipid binding sites.Protein J. 2010 Feb;29(2):127-35. doi: 10.1007/s10930-010-9232-x. Protein J. 2010. PMID: 20195717
-
Involvement of xanthine oxidase and hypoxia-inducible factor 1 in Toll-like receptor 7/8-mediated activation of caspase 1 and interleukin-1β.Cell Mol Life Sci. 2011 Jan;68(1):151-8. doi: 10.1007/s00018-010-0450-3. Epub 2010 Jul 15. Cell Mol Life Sci. 2011. PMID: 20632067 Free PMC article.
-
Mutation of Y179 on phospholipase D2 (PLD2) upregulates DNA synthesis in a PI3K-and Akt-dependent manner.Cell Signal. 2008 Jan;20(1):176-85. doi: 10.1016/j.cellsig.2007.10.009. Epub 2007 Oct 13. Cell Signal. 2008. PMID: 18006275 Free PMC article.
-
Roles of G beta gamma in membrane recruitment and activation of p110 gamma/p101 phosphoinositide 3-kinase gamma.J Cell Biol. 2003 Jan 6;160(1):89-99. doi: 10.1083/jcb.200210115. Epub 2002 Dec 30. J Cell Biol. 2003. PMID: 12507995 Free PMC article.
-
Adsorption of GST-PI3Kgamma at the air-buffer interface and at substrate and nonsubstrate phospholipid monolayers.Biophys J. 2009 Feb;96(3):1016-25. doi: 10.1016/j.bpj.2008.10.032. Biophys J. 2009. PMID: 19186139 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous
