Clasps are CLIP-115 and -170 associating proteins involved in the regional regulation of microtubule dynamics in motile fibroblasts
- PMID: 11290329
- DOI: 10.1016/s0092-8674(01)00288-4
Clasps are CLIP-115 and -170 associating proteins involved in the regional regulation of microtubule dynamics in motile fibroblasts
Abstract
CLIP-170 and CLIP-115 are cytoplasmic linker proteins that associate specifically with the ends of growing microtubules and may act as anti-catastrophe factors. Here, we have isolated two CLIP-associated proteins (CLASPs), which are homologous to the Drosophila Orbit/Mast microtubule-associated protein. CLASPs bind CLIPs and microtubules, colocalize with the CLIPs at microtubule distal ends, and have microtubule-stabilizing effects in transfected cells. After serum induction, CLASPs relocalize to distal segments of microtubules at the leading edge of motile fibroblasts. We provide evidence that this asymmetric CLASP distribution is mediated by PI3-kinase and GSK-3 beta. Antibody injections suggest that CLASP2 is required for the orientation of stabilized microtubules toward the leading edge. We propose that CLASPs are involved in the local regulation of microtubule dynamics in response to positional cues.
Similar articles
-
CLIPs and CLASPs and cellular dynamics.Nat Rev Mol Cell Biol. 2005 Jun;6(6):487-98. doi: 10.1038/nrm1664. Nat Rev Mol Cell Biol. 2005. PMID: 15928712 Review.
-
CLASP1 and CLASP2 bind to EB1 and regulate microtubule plus-end dynamics at the cell cortex.J Cell Biol. 2005 Jan 3;168(1):141-53. doi: 10.1083/jcb.200405094. J Cell Biol. 2005. PMID: 15631994 Free PMC article.
-
CLASPs attach microtubule plus ends to the cell cortex through a complex with LL5beta.Dev Cell. 2006 Jul;11(1):21-32. doi: 10.1016/j.devcel.2006.05.012. Dev Cell. 2006. PMID: 16824950
-
Phosphorylation of CLASP2 by GSK-3beta regulates its interaction with IQGAP1, EB1 and microtubules.J Cell Sci. 2009 Aug 15;122(Pt 16):2969-79. doi: 10.1242/jcs.046649. Epub 2009 Jul 28. J Cell Sci. 2009. PMID: 19638411
-
CLASPs at a glance.J Cell Sci. 2020 Apr 24;133(8):jcs243097. doi: 10.1242/jcs.243097. J Cell Sci. 2020. PMID: 32332092 Free PMC article. Review.
Cited by
-
New alleles of C. elegans gene cls-2 (R107.6), called xc3, xc4, and xc5.MicroPubl Biol. 2017 Dec 19;2017:10.17912/W2RQ2X. doi: 10.17912/W2RQ2X. MicroPubl Biol. 2017. PMID: 32550356 Free PMC article. No abstract available.
-
The leading role of microtubules in endothelial barrier dysfunction: disassembly of peripheral microtubules leaves behind the cytoskeletal reorganization.J Cell Biochem. 2013 Oct;114(10):2258-72. doi: 10.1002/jcb.24575. J Cell Biochem. 2013. PMID: 23606375 Free PMC article.
-
The fission yeast kinetochore component Spc7 associates with the EB1 family member Mal3 and is required for kinetochore-spindle association.Mol Biol Cell. 2004 Dec;15(12):5255-67. doi: 10.1091/mbc.e04-06-0443. Epub 2004 Sep 15. Mol Biol Cell. 2004. PMID: 15371542 Free PMC article.
-
Intracellular control of developmental and regenerative axon growth.Philos Trans R Soc Lond B Biol Sci. 2006 Sep 29;361(1473):1575-92. doi: 10.1098/rstb.2006.1882. Philos Trans R Soc Lond B Biol Sci. 2006. PMID: 16939976 Free PMC article. Review.
-
The yeast ubiquitin protease, Ubp3p, promotes protein stability.Genetics. 2002 Nov;162(3):1079-89. doi: 10.1093/genetics/162.3.1079. Genetics. 2002. PMID: 12454057 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous
