Inhibition of HIV infection by the cytokine midkine
- PMID: 11277697
- DOI: 10.1006/viro.2000.0767
Inhibition of HIV infection by the cytokine midkine
Abstract
The growth factor midkine (MK) has been reported to bind heparan sulfate and nucleolin, two components of the cell surface implicated in the attachment of HIV-1 particles. Here we show that synthetic and recombinant preparations of MK inhibit in a dose-dependent manner infection of cells by T-lymphocyte- and macrophage-tropic HIV-1 isolates. The binding of labeled MK to cells is prevented by excess unlabeled MK or by the anti-HIV pseudopeptide HB-19 that blocks HIV entry by forming a stable complex with the cell-surface-expressed nucleolin. MK mRNA is systematically expressed in adult peripheral blood lymphocytes from healthy donors, while its expression becomes markedly but transiently increased upon in vitro treatment of lymphocytes with IL-2 or IFN-gamma and activation of T-lymphocytes by PHA or antibodies specific to CD3/CD28. In MK-producing lymphocytes, MK is detectable at the cell surface where it colocalizes with the surface-expressed nucleolin. Finally, by using MK-producing CD4(+) and CD4(-) cell clones we show that HIV infection in cell cultures could be inhibited in both an autocrine and a paracrine manner. The potent and distinct anti-HIV action of MK along with its enhanced expression in lymphocytes by various physiological stimuli suggests that MK is a cytokine that could be implicated in HIV-induced pathogenesis.
Copyright 2001 Academic Press.
Similar articles
-
Midkine, a cytokine that inhibits HIV infection by binding to the cell surface expressed nucleolin.Cell Res. 2006 Feb;16(2):174-81. doi: 10.1038/sj.cr.7310024. Cell Res. 2006. PMID: 16474431 Review.
-
Pleiotrophin inhibits HIV infection by binding the cell surface-expressed nucleolin.FEBS J. 2005 Sep;272(18):4646-59. doi: 10.1111/j.1742-4658.2005.04870.x. FEBS J. 2005. PMID: 16156786
-
Complex effects of interferon-alpha on the cytokine network in HIV infection--possible contribution to immunosuppression.Cytokine. 2001 Apr 7;14(1):56-62. doi: 10.1006/cyto.2000.0850. Cytokine. 2001. PMID: 11298493
-
Dendritic cells partially abrogate the regulatory activity of CD4+CD25+ T cells present in the human peripheral blood.Int Immunol. 2007 Mar;19(3):227-37. doi: 10.1093/intimm/dxl139. Epub 2007 Feb 7. Int Immunol. 2007. PMID: 17289657
-
Macrophages, cytokines, and HIV.J Lab Clin Med. 1997 Jan;129(1):10-6. doi: 10.1016/s0022-2143(97)90156-6. J Lab Clin Med. 1997. PMID: 9011585 Review. No abstract available.
Cited by
-
Midkine-A novel player in cardiovascular diseases.Front Cardiovasc Med. 2022 Sep 20;9:1003104. doi: 10.3389/fcvm.2022.1003104. eCollection 2022. Front Cardiovasc Med. 2022. PMID: 36204583 Free PMC article. Review.
-
Targeting MDK Abrogates IFN-γ-Elicited Metastasis inCancers of Various Origins.Front Oncol. 2022 Jun 7;12:885656. doi: 10.3389/fonc.2022.885656. eCollection 2022. Front Oncol. 2022. PMID: 35747815 Free PMC article.
-
Differential Production of Midkine and Pleiotrophin by Innate APCs upon Stimulation through Nucleic Acid-Sensing TLRs.J Immunol Res. 2023 Oct 9;2023:7944102. doi: 10.1155/2023/7944102. eCollection 2023. J Immunol Res. 2023. PMID: 37850119 Free PMC article.
-
Construction of a fusion protein expression vector MK-EGFP and its subcellular localization in different carcinoma cell lines.World J Gastroenterol. 2006 Dec 21;12(47):7649-53. doi: 10.3748/wjg.v12.i47.7649. World J Gastroenterol. 2006. PMID: 17171794 Free PMC article.
-
Transcriptomic analysis of the innate immune response to in vitro transfection of plasmid DNA.Mol Ther Nucleic Acids. 2022 Dec 5;31:43-56. doi: 10.1016/j.omtn.2022.11.025. eCollection 2023 Mar 14. Mol Ther Nucleic Acids. 2022. PMID: 36618265 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
