Role of the ENTH domain in phosphatidylinositol-4,5-bisphosphate binding and endocytosis
- PMID: 11161217
- DOI: 10.1126/science.291.5506.1047
Role of the ENTH domain in phosphatidylinositol-4,5-bisphosphate binding and endocytosis
Abstract
Endocytic proteins such as epsin, AP180, and Hip1R (Sla2p) share a conserved modular region termed the epsin NH2-terminal homology (ENTH) domain, which plays a crucial role in clathrin-mediated endocytosis through an unknown target. Here, we demonstrate a strong affinity of the ENTH domain for phosphatidylinositol-4,5-bisphosphate [PtdIns(4,5)P2]. With nuclear magnetic resonance analysis of the epsin ENTH domain, we determined that a cleft formed with positively charged residues contributed to phosphoinositide binding. Overexpression of a mutant, epsin Lys76 --> Ala76, with an ENTH domain defective in phosphoinositide binding, blocked epidermal growth factor internalization in COS-7 cells. Thus, interaction between the ENTH domain and PtdIns(4,5)P2 is essential for endocytosis mediated by clathrin-coated pits.
Comment in
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Cell biology. A lipid oils the endocytosis machine.Science. 2001 Feb 9;291(5506):993-4. doi: 10.1126/science.291.5506.993. Science. 2001. PMID: 11232585 No abstract available.
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