Sindbis virus replication in vertebrate and mosquito cells: an interpretation
- PMID: 1107684
Sindbis virus replication in vertebrate and mosquito cells: an interpretation
Abstract
This paper summarizes recent comparative studies of Sindbis virus (SV) replication in cultured Aedes albopictus (A. albo) or (A. aegypti (A. aeg) and BHK21 or chick embryo (CEF) cells. 1. Viral growth kinetics and yields are similar in A. albo cells at 28 degress C and in vertebrate cells at 37 degrees C. A. albo exhibit no CPE and yield persistenetly infected cultures. 2. SV grown in A. albo cells lacks sialic acid but is antigenically and in terms of particle/PFU or particle/HAU ratios equivalent to SV derived from vertebrate cells. The contrast to VSV in the latter respect is discussed. 3. SV from persistently infected A. albo or A. aeg cells is temperature-sensitive, thermolabile, and produces small plaques. Partial characterization of these mutants, of RNA associated with their replication, and their high reversion rate to ts+ upon serial undiluted passage in GHK21 cells are presented. 4. Hostdependent differences in the generation of defective-interfering (DI) SV particles and of low molecular weight viral RNA species have been observed upon undiluted serial passages in BHK21 and CEF. In contrast, serial passage in A. albo cells appears not to produce DI particles or small RNA species nor do these cells "recognize" as such DI particles from BHK21 cells. 5. Possible implications of these observations fro the natural life cycle of arthropod-borne togaviruses are discussed.
Similar articles
-
Properties of defective interfering particles of Sindbis virus generated in vertebrate and mosquito cells.J Gen Virol. 1984 Feb;65 ( Pt 2):333-41. doi: 10.1099/0022-1317-65-2-333. J Gen Virol. 1984. PMID: 6693857
-
The significance and nature of defective interfering viruses.Bull Schweiz Akad Med Wiss. 1977 Sep;33(4-6):229-42. Bull Schweiz Akad Med Wiss. 1977. PMID: 912148
-
Effect of incubation time on the generation of defective-interfering particles during undiluted serial passage of sindbis virus in Aedes albopictus and chick cells.Virology. 1979 Jul 15;96(1):229-38. doi: 10.1016/0042-6822(79)90186-7. Virology. 1979. PMID: 462807 No abstract available.
-
Growth cycle of arboviruses in vertebrate and arthropod cells.Prog Med Virol. 1975;19:257-323. Prog Med Virol. 1975. PMID: 164052 Review. No abstract available.
-
Insect-transmitted vertebrate viruses: alphatogaviruses.In Vitro Cell Dev Biol Anim. 1993 Apr;29A(4):289-95. doi: 10.1007/BF02633957. In Vitro Cell Dev Biol Anim. 1993. PMID: 8320181 Review.
Cited by
-
Arboviruses: persistence and defectiveness in Sindbis virus infections.Med Microbiol Immunol. 1977;164(1-3):77-85. doi: 10.1007/BF02121304. Med Microbiol Immunol. 1977. PMID: 600205 No abstract available.
-
Failure of defective interfering particles of Sindbis virus produced in BHK or chicken cells to affect viral replication in Aedes albopictus cells.J Virol. 1976 Aug;19(2):398-408. doi: 10.1128/JVI.19.2.398-408.1976. J Virol. 1976. PMID: 986481 Free PMC article.
-
Rapid selection against arbovirus-induced apoptosis during infection of a mosquito vector.Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):E1152-61. doi: 10.1073/pnas.1424469112. Epub 2015 Feb 23. Proc Natl Acad Sci U S A. 2015. PMID: 25713358 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources
Research Materials
Miscellaneous