doi: 10.1021/bi00692a011.
A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography
- PMID: 1101952
- DOI: 10.1021/bi00692a011
Item in Clipboard
A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography
Biochemistry.
.
Abstract
An improved method is described for the purification of the DNA-dependent RNA polymerase [ribonucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6] from Escherichia coli. The method involves lysozyme-sodium deoxycholate lysis, low-speed centrifugation, precipitation with Polymin P, elution from the Polymin P precipitate, ammonium sulfate precipitation, and chromatography on DNA-cellulose and Bio-Gel A 5m. RNA polymerase is purified to electrophoretic homogeneity in 2 days with a recovery of 45%, resulting in a yield of 250 mg of holoenzyme from 500 g of cells.
Similar articles
-
A new method for the large-scale purification of wheat germ DNA-dependent RNA polymerase II.Biochemistry. 1975 Oct 21;14(21):4639-45. doi: 10.1021/bi00692a012. Biochemistry. 1975. PMID: 1182107
-
Purification of DNA-dependent RNA polymerase from Streptomyces granaticolor.Folia Microbiol (Praha). 1983;28(5):435-8. doi: 10.1007/BF02879496. Folia Microbiol (Praha). 1983. PMID: 6642321
-
A procedure for the rapid purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase.Biochem J. 1973 May;133(1):201-3. doi: 10.1042/bj1330201. Biochem J. 1973. PMID: 4578761 Free PMC article.
-
[Improved method of E. coli RNA polymerase purification].Tanpakushitsu Kakusan Koso. 1972 Oct:Suppl:133-9. Tanpakushitsu Kakusan Koso. 1972. PMID: 4567703 Review. Japanese. No abstract available.
-
[Purification of E. coli RNA polymerase].Tanpakushitsu Kakusan Koso. 1972 Oct:Suppl:127-33. Tanpakushitsu Kakusan Koso. 1972. PMID: 4567702 Review. Japanese. No abstract available.
Cited by
-
Direct interactions between the coiled-coil tip of DksA and the trigger loop of RNA polymerase mediate transcriptional regulation.Genes Dev. 2012 Dec 1;26(23):2634-46. doi: 10.1101/gad.204693.112. Genes Dev. 2012. PMID: 23207918 Free PMC article.
-
Effect of DNA bending in various regions of a consensus-like Escherichia coli promoter on its strength in vivo and structure of the open complex in vitro.Nucleic Acids Res. 1991 Jun 11;19(11):2947-53. doi: 10.1093/nar/19.11.2947. Nucleic Acids Res. 1991. PMID: 2057353 Free PMC article.
-
A specific and efficient photoreaction between E. coli RNA polymerase and T+1 in the lacUV5 or deoP1 promoter.Nucleic Acids Res. 1988 Oct 25;16(20):9545-55. doi: 10.1093/nar/16.20.9545. Nucleic Acids Res. 1988. PMID: 3054808 Free PMC article.
-
Controlled protein release from polyethyleneimine-coated poly(L-lactic acid)/pluronic blend matrices.Pharm Res. 1992 Jan;9(1):37-9. doi: 10.1023/a:1018971525301. Pharm Res. 1992. PMID: 1589407
-
Pyrophosphate-condensing activity linked to nucleic acid synthesis.Nucleic Acids Res. 1979 Apr;6(4):1521-34. doi: 10.1093/nar/6.4.1521. Nucleic Acids Res. 1979. PMID: 88040 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources
Research Materials