[Expression and characterization of HIV-1 Gag p17-p24 protein]
[Article in
Chinese]
Affiliations
- PMID: 10883279
Item in Clipboard
[Expression and characterization of HIV-1 Gag p17-p24 protein]
[Article in
Chinese]
Sheng Wu Gong Cheng Xue Bao.
2000 Jan.
Abstract
The biological and immunological characteristics of HIV-1 core protein p17-24 expressed by recombinant vaccinia viruses were studied. The results of indirect immunofluorescence assay (I-IFA), Western blot and dot ELISA showed that the two recombinants could express the p24 gag and p17-24 gag fusion proteins in infected cell lines respectively. The electromicroscope observation revealed that the expressed proteins could also assemble into virus-like particles. The recombinant vaccinia viruses can also stimulate mice for the formation of anti HIV-1 Gag p24 antibody. When infected with the recombinant viruses, the chromosome DNA ladder caused by the apoptosis of the BHK cell was observed.
Similar articles
-
Enhancement of cellular and humoral immune responses to human immunodeficiency virus type 1 Gag and Pol by a G/P-92 fusion protein expressing highly immunogenic Gag p17/p24 and Pol p51 antigens.J Hum Virol. 2001 Nov-Dec;4(6):306-16. J Hum Virol. 2001. PMID: 12082397
-
Immunization of human HIV-seronegative volunteers with recombinant p17/p24:Ty virus-like particles elicits HIV-1 p24-specific cellular and humoral immune responses.AIDS. 1993 Oct;7(10):1315-23. AIDS. 1993. PMID: 8267904
-
Morphogenic capabilities of human immunodeficiency virus type 1 gag and gag-pol proteins in insect cells.Virology. 1993 Mar;193(1):242-55. doi: 10.1006/viro.1993.1120. Virology. 1993. PMID: 8438569
-
[Expression of HIV-1 tat gene under the control of P 7,5 KD vaccinia virus promoter in CV-1 cells].Vestn Ross Akad Med Nauk. 1992;(9-10):52-5. Vestn Ross Akad Med Nauk. 1992. PMID: 1283722 Review. Russian.
-
[Anti-HIV-1 p17 antibody as anti-HIV agent].Nihon Rinsho. 1993 Sep;51 Suppl:213-8. Nihon Rinsho. 1993. PMID: 8271386 Review. Japanese. No abstract available.