Properties of two VEGF receptors, Flt-1 and KDR, in signal transduction
- PMID: 10865839
- DOI: 10.1111/j.1749-6632.2000.tb06314.x
Properties of two VEGF receptors, Flt-1 and KDR, in signal transduction
Abstract
The properties of two VEGF receptors, Flt-1 and KDR, in the signal transduction of VEGF in human umbilical vein endothelial cells (HUVECs) were investigated by using two newly developed blocking monoclonal antibodies (mAbs) against Flt-1 and KDR. VEGF stimulated the expression of transcription factor Ets-1 as well as matrix metalloproteinase-1 (MMP-1) and Flt-1 in HUVECs. The KDR/Flt-1 heterodimer and the KDR homodimer mediate the expression of Ets-1, MMP-1, and Flt-1. VEGF also stimulated DNA synthesis and migration of HUVECs. DNA synthesis is mediated by the same signaling system as the expression of Ets-1. In contrast, cell migration is regulated by two distinct signaling systems. The Flt-1 homodimer is required for actin reorganization. The KDR/Flt-1 heterodimer and the KDR homodimer are required for the assembly of vinculin in focal adhesion plaque by regulating the phosphorylation of focal adhesion kinase (FAK) and paxillin.
Similar articles
-
Roles of two VEGF receptors, Flt-1 and KDR, in the signal transduction of VEGF effects in human vascular endothelial cells.Oncogene. 2000 Apr 20;19(17):2138-46. doi: 10.1038/sj.onc.1203533. Oncogene. 2000. PMID: 10815805
-
Signal transduction and transcriptional regulation of angiogenesis.Adv Exp Med Biol. 2000;476:109-15. doi: 10.1007/978-1-4615-4221-6_9. Adv Exp Med Biol. 2000. PMID: 10949659
-
Possible involvement of VEGF-FLT tyrosine kinase receptor system in normal and tumor angiogenesis.Princess Takamatsu Symp. 1994;24:162-70. Princess Takamatsu Symp. 1994. PMID: 8983073 Review.
-
Analysis of biological effects and signaling properties of Flt-1 (VEGFR-1) and KDR (VEGFR-2). A reassessment using novel receptor-specific vascular endothelial growth factor mutants.J Biol Chem. 2001 Feb 2;276(5):3222-30. doi: 10.1074/jbc.M002016200. Epub 2000 Oct 31. J Biol Chem. 2001. PMID: 11058584
-
Role of VEGF-flt receptor system in normal and tumor angiogenesis.Adv Cancer Res. 1995;67:281-316. doi: 10.1016/s0065-230x(08)60716-2. Adv Cancer Res. 1995. PMID: 8571818 Review. No abstract available.
Cited by
-
The KDR Gene rs2071559 and the VEGF Gene rs6921438 May Be Associated with Diabetic Nephropathy in Caucasians with Type 2 Diabetes Mellitus.Int J Mol Sci. 2024 Aug 30;25(17):9439. doi: 10.3390/ijms25179439. Int J Mol Sci. 2024. PMID: 39273385 Free PMC article.
-
Bioinformatics to Identify Biomarkers of Diabetic Nephropathy based on Sphingolipid Metabolism and their Molecular Mechanisms.Curr Diabetes Rev. 2024;21(2):e070524229720. doi: 10.2174/0115733998297749240418071555. Curr Diabetes Rev. 2024. PMID: 38712372
-
Single-cell analysis of uterosacral ligament revealed cellular heterogeneity in women with pelvic organ prolapse.Commun Biol. 2024 Feb 7;7(1):159. doi: 10.1038/s42003-024-05808-3. Commun Biol. 2024. PMID: 38326542 Free PMC article.
-
Prediction of preeclampsia throughout gestation with maternal characteristics and biophysical and biochemical markers: a longitudinal study.Am J Obstet Gynecol. 2022 Jan;226(1):126.e1-126.e22. doi: 10.1016/j.ajog.2021.01.020. Epub 2021 Apr 16. Am J Obstet Gynecol. 2022. PMID: 34998477 Free PMC article.
-
Integrated Analysis of Three Publicly Available Gene Expression Profiles Identified Genes and Pathways Associated with Clear Cell Renal Cell Carcinoma.Med Sci Monit. 2020 Jul 26;26:e919965. doi: 10.12659/MSM.919965. Med Sci Monit. 2020. PMID: 32712616 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous
