doi: 10.1006/meth.2000.0987.
Assays for functional binding of plasminogen to viral proteins
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- PMID: 10816377
- DOI: 10.1006/meth.2000.0987
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Assays for functional binding of plasminogen to viral proteins
Methods.
2000 Jun.
Abstract
Cleavage of the hemagglutinin (HA) molecule by proteases is a prerequisite for the infectivity of influenza A viruses. Plasminogen binds to the viral glycoprotein neuraminidase (NA), and NA-bound plasminogen is activated to plasmin, which cleaves the HA of influenza A/WSN/33 (WSN) (H1N1) virus. Here we present assays for detecting functional plasminogen binding to the influenza virus NA.
Copyright 2000 Academic Press.
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