Suppression of in vitro antibody synthesis by immunoglobulin-binding factor
- PMID: 1079850
- PMCID: PMC2189901
- DOI: 10.1084/jem.142.2.507
Suppression of in vitro antibody synthesis by immunoglobulin-binding factor
Abstract
Alloantigen-activated mouse T cells secrete a factor which binds to the Fc fragment of IgG and blocks complement (C) activation by IgG (immunoglobulin-binding factor, IBF). IBF was found to suppress the direct plaque-forming cell (PFC) response of mouse spleen cell cultures to sheep erythrocytes and to dinitrophenylated aminoethyldextran (T-independent antigen). Purification of IBF by affinity chromatography on IgG-coated Sepharose columns led to an increase of the suppressive capacity with IgG, IgM, or Fab2 from IgG) the factor responsible for inhibiting the PFC response could not be dissociated from that responsible for the inhibitory activity of IBF on C-dependent hemolysis. No effect was seen when cultures were pretreated for 6 h, or when IBF was added at 72 h. These data are compatible with the view that IBF is a soluable mediator of suppressor T cells which may interfere with terminal differentiation of antibody-forming cell precursors.
Similar articles
-
Characterization of suppressive immunoglobulin-binding factor (IBF). I. Production of IBF by a theta-positive lymphoma (L-5178-Y).J Immunol. 1977 Oct;119(4):1266-8. J Immunol. 1977. PMID: 302279
-
Regulation of antibody synthesis to sheep red cells in cultures of nu/nu spleen cells by T-cell replacing factor (TRF) and immunoglobulin-binding factor (IBF).Ann Immunol (Paris). 1979 Sep-Oct;130C(5):711-20. Ann Immunol (Paris). 1979. PMID: 316681
-
Immune responses in vitro. XI. Suppression of primary IgM and IgG plaque-forming cell responses in vitro by alloantisera against leukocyte alloantigens.J Exp Med. 1974 Oct 1;140(4):921-38. doi: 10.1084/jem.140.4.921. J Exp Med. 1974. PMID: 4610076 Free PMC article.
-
Reversible binding of a T cell factor on IgG-coated sepharose beads.Eur J Immunol. 1976 Jan;6(1):69-72. doi: 10.1002/eji.1830060115. Eur J Immunol. 1976. PMID: 971721
-
Hapten-IgG-induced suppression of the in vitro antibody response: role of hapten density and of antigenic challenge.Cell Immunol. 1977 Mar 1;29(1):72-85. doi: 10.1016/0008-8749(77)90276-3. Cell Immunol. 1977. PMID: 321138 Review. No abstract available.
Cited by
-
Nature of the antigenic complex recognized by T lymphocytes: specific sensitization by antigens associated with allogeneic macrophages.Proc Natl Acad Sci U S A. 1977 May;74(5):2104-8. doi: 10.1073/pnas.74.5.2104. Proc Natl Acad Sci U S A. 1977. PMID: 68474 Free PMC article.
-
IgE class-restricted tolerance induced by neonatal administration of soluble or cell-bound IgE. Cellular mechanisms.J Exp Med. 1984 Oct 1;160(4):953-70. doi: 10.1084/jem.160.4.953. J Exp Med. 1984. PMID: 6237166 Free PMC article.
-
Fc-receptor-bearing cells in spleen of mice injected with cell-free Ehrlich ascites fluid (EAF).Blut. 1980 Oct;41(4):269-76. doi: 10.1007/BF01020527. Blut. 1980. PMID: 6448644
-
Identification of soluble Fc receptors in mouse serum and the conditioned medium of stimulated B cells.J Exp Med. 1984 Dec 1;160(6):1836-49. doi: 10.1084/jem.160.6.1836. J Exp Med. 1984. PMID: 6239903 Free PMC article.
-
Inhibitory mechanism of the proliferative responses of resting B cells: feedback regulation by a lymphokine (suppressive B-cell factor) produced by Fc receptor-stimulated B cells.Immunology. 1987 May;61(1):35-41. Immunology. 1987. PMID: 2953673 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous
