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. 2000 May 15;348 Pt 1(Pt 1):107-12.

pp60c-src associates with the SH2-containing inositol-5-phosphatase SHIP1 and is involved in its tyrosine phosphorylation downstream of alphaIIbbeta3 integrin in human platelets

S Giuriato  1 , S Bodin, C Erneux, R Woscholski, M Plantavid, H Chap, B Payrastre
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pp60c-src associates with the SH2-containing inositol-5-phosphatase SHIP1 and is involved in its tyrosine phosphorylation downstream of alphaIIbbeta3 integrin in human platelets

S Giuriato et al. Biochem J. .

Abstract

SH2-containing inositol-5-phosphatase 1 (SHIP1) was originally identified as a 145 kDa protein that became tyrosine-phosphorylated in response to multiple cytokines. It is now well established that SHIP1 is specifically expressed in haemopoietic cells and is important as a negative regulator of signalling. We found recently that SHIP1 was present in human blood platelets as an Ins(1,3,4, 5)P(4)-phosphatase and a PtdIns(3,4,5)P(3)-5-phosphatase that became tyrosine-phosphorylated and was relocated to the cytoskeleton in an integrin-dependent manner. Here we report biochemical and pharmacological evidence that the tyrosine kinase pp60(c-src) is constitutively associated with SHIP1 and is involved in its tyrosine phosphorylation downstream of integrin engagement in thrombin-activated human platelets. The use of cytochalasin D allowed us to demonstrate that the actin cytoskeleton reorganization induced on thrombin stimulation was not required for its integrin-mediated phosphorylation. Moreover, the integrin-dependent relocation of SHIP1 to the cytoskeleton did not require its tyrosine phosphorylation. These results suggest that SHIP1 is first recruited to the integrin-linked signalling complexes and then becomes tyrosine-phosphorylated through a Src-kinase-dependent mechanism but independently of the actin cytoskeleton reorganization.

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References

    1. Blood. 1999 Dec 15;94(12):4156-65 - PubMed
    1. Mol Cell. 1999 May;3(5):639-48 - PubMed
    1. Proc Natl Acad Sci U S A. 1989 Feb;86(3):901-5 - PubMed
    1. Proc Natl Acad Sci U S A. 1989 Apr;86(7):2234-8 - PubMed
    1. J Biol Chem. 1991 Dec 15;266(35):23554-7 - PubMed

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