Distinct protein interaction domains and protein spreading in a complex centromere
- PMID: 10766735
- PMCID: PMC316498
Distinct protein interaction domains and protein spreading in a complex centromere
Abstract
Fission yeast (Schizosaccharomyces pombe) centromeres are composed of large (40-100 kb) inverted repeats that display heterochromatic features, thus providing a good model for higher eukaryotic centromeres. The association of three proteins that mediate region-specific silencing across centromere 1 has been mapped by quantitative chromatin immunoprecipitation. Swi6 and Chp1 are confined to the flanking outer repeats and Swi6 can spread across at least 3 kb of extraneous chromatin in cen1. In contrast, Mis6 coats the inner repeats and central core. tRNA genes demarcate this transition zone. These analyses clearly define two distinct domains within this complex centromere which interact with different proteins.
Figures





Similar articles
-
Distinct centromere domain structures with separate functions demonstrated in live fission yeast cells.J Cell Sci. 2003 Oct 1;116(Pt 19):4035-42. doi: 10.1242/jcs.00707. Epub 2003 Aug 19. J Cell Sci. 2003. PMID: 12928332
-
Requirement of heterochromatin for cohesion at centromeres.Science. 2001 Dec 21;294(5551):2539-42. doi: 10.1126/science.1064027. Epub 2001 Oct 11. Science. 2001. PMID: 11598266
-
Recruitment of cohesin to heterochromatic regions by Swi6/HP1 in fission yeast.Nat Cell Biol. 2002 Jan;4(1):89-93. doi: 10.1038/ncb739. Nat Cell Biol. 2002. PMID: 11780129
-
Centromere structure and function in budding and fission yeasts.New Biol. 1990 Jan;2(1):10-9. New Biol. 1990. PMID: 2078550 Review.
-
Studies on the mechanism of RNAi-dependent heterochromatin assembly.Cold Spring Harb Symp Quant Biol. 2006;71:461-71. doi: 10.1101/sqb.2006.71.044. Cold Spring Harb Symp Quant Biol. 2006. PMID: 17381328 Review.
Cited by
-
Intrinsic Toxicity of Unchecked Heterochromatin Spread Is Suppressed by Redundant Chromatin Boundary Functions in Schizosacchromyces pombe.G3 (Bethesda). 2015 May 8;5(7):1453-61. doi: 10.1534/g3.115.018663. G3 (Bethesda). 2015. PMID: 25957277 Free PMC article.
-
γH2A-binding protein Brc1 affects centromere function in fission yeast.Mol Cell Biol. 2013 Apr;33(7):1410-6. doi: 10.1128/MCB.01654-12. Epub 2013 Jan 28. Mol Cell Biol. 2013. PMID: 23358415 Free PMC article.
-
A chromodomain protein, Chp1, is required for the establishment of heterochromatin in fission yeast.EMBO J. 2004 Oct 1;23(19):3825-35. doi: 10.1038/sj.emboj.7600401. Epub 2004 Sep 16. EMBO J. 2004. PMID: 15372076 Free PMC article.
-
Ribonuclease activity of Dis3 is required for mitotic progression and provides a possible link between heterochromatin and kinetochore function.PLoS One. 2007 Mar 21;2(3):e317. doi: 10.1371/journal.pone.0000317. PLoS One. 2007. PMID: 17380189 Free PMC article.
-
The Schizosaccharomyces pombe HIRA-like protein Hip1 is required for the periodic expression of histone genes and contributes to the function of complex centromeres.Mol Cell Biol. 2004 May;24(10):4309-20. doi: 10.1128/MCB.24.10.4309-4320.2004. Mol Cell Biol. 2004. PMID: 15121850 Free PMC article.
References
-
- Aagaard L, Laible G, Selenko P, Schmid M, Dorn R, Schotta G, Kuhfittig S, Wolf A, Lebersorger A, Singh PB, et al. Functional mammalian homologues of the Drosophila PEV modifier Su(var)3-9 encode centromere-associated proteins which complex with the heterochromatin component M31. EMBO J. 1999;18:1923–1938. - PMC - PubMed
-
- Allshire RC. Epigenetic mechanisms of gene regulation. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press; 1996. Transcriptional silencing in the fission yeast: A manifestation of higher order chromosome structure and functions; pp. 443–466.
-
- Allshire RC, Javerzat J-P, Redhead NJ, Cranston G. Position effect variegation at fission yeast centromeres. Cell. 1994;76:157–169. - PubMed
-
- Allshire RC, Nimmo ER, Ekwall K, Javerzat J-P, Cranston G. Mutations derepressing silent domains within fission yeast centromeres disrupt chromosome segregation. Genes & Dev. 1995;9:218–233. - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases