Herpesvirus papio 2: alternative antigen for use in monkey B virus diagnostic assays
- PMID: 10638495
Herpesvirus papio 2: alternative antigen for use in monkey B virus diagnostic assays
Abstract
Background and purpose: Serologic testing for antibody to monkey B virus (BV) in macaque sera is problematic due to the biohazardous nature of BV and BV antigens. Herpesvirus papio 2 (HVP2), a herpesvirus of baboons, is more closely related genetically and antigenically to BV than is human herpes simplex virus 1 (HSV1). The potential for use of HVP2 relative to HSV1 as an alternative test antigen for detection of anti-BV antibody in macaque sera was assessed.
Methods: Standard ELISA formats were developed, using BV-, HVP2-, and HSV1-infected cell extracts. Performance of the HVP2 and HSV1 tests was assessed relative to that of the BV test.
Results: Using the BV antigen ELISA, 349 sera from 7 macaque species were tested, and results were classified as positive (253), negative (94), or suspect (2). The ELISA using HVP2 antigen detected 98.0% of BV-positive sera (248 of 253), whereas the HSV1-based ELISA detected only 96.0% (243 of 253). All three ELISAs identified the same two samples as suspect, and the HSV1 ELISA identified three additional BV-positive sera as suspect.
Conclusions: The HVP2 antigen-based ELISA was equal in sensitivity and specificity to the BV antigen-based ELISA and was superior to the HSV1 ELISA for detection of BV-positive macaque sera. In addition, the HVP2 ELISA has greater laboratory safety, compared with BV antigen use for ELISA testing.
Similar articles
-
Validation of an enzyme-linked immunosorbent assay kit using herpesvirus papio 2 (HVP2) antigen for detection of herpesvirus simiae (B virus) infection in rhesus monkeys.Comp Med. 2005 Jun;55(3):244-8. Comp Med. 2005. PMID: 16089172
-
Prevalence of Herpesvirus papio 2 in baboons and identification of immunogenic viral polypeptides.Lab Anim Sci. 1997 Jun;47(3):256-62. Lab Anim Sci. 1997. PMID: 9241626
-
Use of herpesvirus papio 2 as an alternative antigen in immunoblotting assay for B virus diagnosis.J Vet Med Sci. 2004 May;66(5):529-32. doi: 10.1292/jvms.66.529. J Vet Med Sci. 2004. PMID: 15187363
-
[Pathogenesis of animal herpesviruses to human].Nihon Rinsho. 2000 Apr;58(4):957-61. Nihon Rinsho. 2000. PMID: 10774223 Review. Japanese.
-
Herpesvirus infections of laboratory macaques.J Immunotoxicol. 2010 Apr-Jun;7(2):102-13. doi: 10.3109/15476910903409843. J Immunotoxicol. 2010. PMID: 19995244 Review.
Cited by
-
Poisson factor models with applications to non-normalized microRNA profiling.Bioinformatics. 2013 May 1;29(9):1105-11. doi: 10.1093/bioinformatics/btt091. Epub 2013 Feb 21. Bioinformatics. 2013. PMID: 23428639 Free PMC article.
-
Simian T-lymphotropic Virus-associated lymphoma in 2 naturally infected baboons: T-cell clonal expansion and immune response during tumor development.Comp Med. 2013 Jun;63(3):288-94. Comp Med. 2013. PMID: 23759532 Free PMC article.
-
Reassessing the detection of B-virus-specific serum antibodies.Comp Med. 2012 Dec;62(6):516-26. Comp Med. 2012. PMID: 23561886 Free PMC article.
-
Simultaneous detection of antibodies to five simian viruses in nonhuman primates using recombinant viral protein based multiplex microbead immunoassays.J Virol Methods. 2011 Dec;178(1-2):143-52. doi: 10.1016/j.jviromet.2011.09.004. Epub 2011 Sep 17. J Virol Methods. 2011. PMID: 21945221 Free PMC article.
-
Macacine Herpesvirus 1 in Long-Tailed Macaques, Malaysia, 2009-2011.Emerg Infect Dis. 2015 Jul;21(7):1107-13. doi: 10.3201/eid2107.140162. Emerg Infect Dis. 2015. PMID: 26080081 Free PMC article.