Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1976 Jan;73(1):19-23.
doi: 10.1073/pnas.73.1.19.

Inhibition of initiation of protein synthesis by 7-methylguanosine-5'-monophosphate

E D Hickey, L A Weber, C Baglioni

Inhibition of initiation of protein synthesis by 7-methylguanosine-5'-monophosphate

E D Hickey et al. Proc Natl Acad Sci U S A. 1976 Jan.

Abstract

Translation of rabbit globin mRNA in a wheat germ protein-synthesizing system is inhibited by the nucleotide 7-methylguanosine-5'-monophosphate (m7G5'p) but not by other guanosine nucleotides without the 7-methyl group or with the phosphate in a different position. Translation of RNA of tobacco mosaic virus and poly(A) + HeLa RNA is also inhibited by m7G5'p. We show that m7G5'p prevents the association of mRNA with ribosomal subunits to form an initiation complex. We propose that m7G5'p interacts with a site on initiation factor(s) or ribosomes which is involved in mRNA recognition, presumably by binding to the 5'-terminal sequence m7G5'ppp. m7G5'p does not inhibit translation of poly(U) and RNA of satellite tobacco necrosis virus, which do not have the 5'-terminal sequence m7G5'ppp. In the case of RNA of satellite tobacco necrosis virus, some stimulation of its translation is consistently observed in the presence of m7G5'p; possible interpretations of this finding are discussed.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1189-93 - PubMed
    1. Nature. 1975 May 1;255(5503):33-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1975 Jan;72(1):318-22 - PubMed
    1. Nucleic Acids Res. 1975 Jul;2(7):1189-201 - PubMed
    1. FEBS Lett. 1975 Sep 1;57(1):31-4 - PubMed

Publication types

LinkOut - more resources