Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1975 Sep;72(9):3628-32.
doi: 10.1073/pnas.72.9.3628.

Transposition of R factor genes to bacteriophage lambda

D E Berg, J Davies, B Allet, J D Rochaix

Transposition of R factor genes to bacteriophage lambda

D E Berg et al. Proc Natl Acad Sci U S A. 1975 Sep.

Abstract

Transpositions of segments of R factor (antibiotic resistance plasmids) to bacteriophage lambda have been selected and characterized. Cells of Escherichia coli harboring R factors that determine kanamycin resistance were infected with phage lambda, and lambdakan transducing lines were obtained. Each of the three examined is unusual when compared to lambda transducing phages containing E. coli chromosomal genes: the kan insertions (a) occur at several sites, each well removed from the integration region POP', (b) are not associated with deletion of lambda phage DNA, and (c) are separable from the lambda genome during transduction or during lytic growth. Two insertions from the same R factor contain 1.5 kilobase sequences repeated in inverted order. The properties of the lambdakan phage suggest that R factors contain systems capable of mediating genetic exchange in the absence of extensive DNA homology. It is suggested that such systems of exchange may have played important roles in R factor evolution.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Bacteriol. 1964 Nov;88:1266-76 - PubMed
    1. Bacteriol Rev. 1972 Dec;36(4):504-24 - PubMed
    1. J Mol Biol. 1974 Jan 5;85(4):475-84 - PubMed
    1. J Biol Chem. 1970 Dec 25;245(24):6683-9 - PubMed
    1. Bacteriol Rev. 1972 Sep;36(3):361-405 - PubMed

LinkOut - more resources