Endocytosis and vacuolar morphology in Saccharomyces cerevisiae are altered in response to ethanol stress or heat shock
- PMID: 10487923
- DOI: 10.1002/(SICI)1097-0061(19990915)15:12<1211::AID-YEA448>3.0.CO;2-H
Endocytosis and vacuolar morphology in Saccharomyces cerevisiae are altered in response to ethanol stress or heat shock
Abstract
The vital lipophilic dye N-(3-triethylammoniumpropyl)-4-[6-(4-(diethylamino)phenyl]hexatrie nyl ) pyridinium dibromide (FM 4-64) was used to study the effect of ethanol stress and heat shock on endocytosis in the yeast Saccharomyces cerevisiae. Yeast cells stained with FM 4-64 were placed in a culture chamber and the internalization of the dye was monitored by fluorescence microscopy during perfusion of the cells with fresh growth medium. In the absence of ethanol in the perfusion medium, the internalization of FM 4-64 from the plasma membrane to the vacuolar membrane by yeast cells harvested from the exponential phase of growth was completed in 30 min. The presence of 6% (v/v) ethanol in the perfusion medium had no obvious effect on the internalization of FM 4-64 from the plasma membrane, but did lead to an accumulation of the dye in endocytic intermediates. Consequently, vacuolar membrane staining was delayed. Cells stained with FM 4-64 and subjected to heat shock displayed a similar effect, with endocytic intermediates becoming more prominent with the severity of the heat shock. For both ethanol stress and heat shock, vacuolar morphology altered from segregated structures to a single, large organelle. The findings of this study reinforce previous observations that ethanol stress and heat shock induce similar responses in yeast.
Copyright 1999 John Wiley & Sons, Ltd.
Similar articles
-
A new vital stain for visualizing vacuolar membrane dynamics and endocytosis in yeast.J Cell Biol. 1995 Mar;128(5):779-92. doi: 10.1083/jcb.128.5.779. J Cell Biol. 1995. PMID: 7533169 Free PMC article.
-
Vacuolar functions are involved in stress-protective effect of intracellular proline in Saccharomyces cerevisiae.J Biosci Bioeng. 2005 Nov;100(5):538-44. doi: 10.1263/jbb.100.538. J Biosci Bioeng. 2005. PMID: 16384793
-
Isolation of yeast mutants defective for localization of vacuolar vital dyes.Proc Natl Acad Sci U S A. 1998 Sep 29;95(20):11721-6. doi: 10.1073/pnas.95.20.11721. Proc Natl Acad Sci U S A. 1998. PMID: 9751732 Free PMC article.
-
Tolerance and stress response to ethanol in the yeast Saccharomyces cerevisiae.Appl Microbiol Biotechnol. 2009 Nov;85(2):253-63. doi: 10.1007/s00253-009-2223-1. Epub 2009 Sep 16. Appl Microbiol Biotechnol. 2009. PMID: 19756577 Review.
-
FM-dyes as experimental probes for dissecting vesicle trafficking in living plant cells.J Microsc. 2004 May;214(Pt 2):159-73. doi: 10.1111/j.0022-2720.2004.01348.x. J Microsc. 2004. PMID: 15102063 Review.
Cited by
-
Single-cell analysis of S. cerevisiae growth recovery after a sublethal heat-stress applied during an alcoholic fermentation.J Ind Microbiol Biotechnol. 2011 Jun;38(6):687-96. doi: 10.1007/s10295-010-0814-6. Epub 2010 Aug 24. J Ind Microbiol Biotechnol. 2011. PMID: 20734106
-
Accelerated invagination of vacuoles as a stress response in chronically heat-stressed yeasts.Sci Rep. 2018 Feb 8;8(1):2644. doi: 10.1038/s41598-018-20781-8. Sci Rep. 2018. PMID: 29422608 Free PMC article.
-
Comprehensive transcriptome analysis of faba bean in response to vernalization.Planta. 2019 Nov 28;251(1):22. doi: 10.1007/s00425-019-03308-x. Planta. 2019. PMID: 31781953
-
Heat Stress-Dependent Association of Membrane Trafficking Proteins With mRNPs Is Selective.Front Plant Sci. 2021 Jun 24;12:670499. doi: 10.3389/fpls.2021.670499. eCollection 2021. Front Plant Sci. 2021. PMID: 34249042 Free PMC article.
-
Protective Effects of Arginine on Saccharomyces cerevisiae Against Ethanol Stress.Sci Rep. 2016 Aug 10;6:31311. doi: 10.1038/srep31311. Sci Rep. 2016. PMID: 27507154 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases