Antiviral drug susceptibility assays: going with the flow
- PMID: 10480260
- DOI: 10.1016/s0166-3542(99)00039-x
Antiviral drug susceptibility assays: going with the flow
Abstract
This review describes the procedures for the use of fluorochrome labeled monoclonal antibodies and flow cytometry for the detection and quantification of virus infected cells. The application of this technology for (1) identifying virus infected cells in clinical specimens obtained from human cytomegalovirus (HCMV) and human immunodeficiency virus (HIV) infected individuals; (2) screening antiviral compounds active against HCMV, HDSV and HIV; and (3) performing drug susceptibility testing for HCMV, HSV and HIV clinical isolates are reviewed. The flow cytometry drug susceptibility assay is rapid, quantitative, and easily performed. It should be considered by anyone interested in performing drug susceptibility testing for any virus for which there are reliable monoclonal antibodies.
Similar articles
-
Novel Method Based on Real-Time Cell Analysis for Drug Susceptibility Testing of Herpes Simplex Virus and Human Cytomegalovirus.J Clin Microbiol. 2016 Aug;54(8):2120-7. doi: 10.1128/JCM.03274-15. Epub 2016 Jun 1. J Clin Microbiol. 2016. PMID: 27252463 Free PMC article.
-
Assays for antiviral drug resistance.Antiviral Res. 1995 Apr;26(4):403-13. doi: 10.1016/0166-3542(95)00028-k. Antiviral Res. 1995. PMID: 7574542 Review. No abstract available.
-
The use of flow cytometry to detect antiviral resistance in human cytomegalovirus.J Virol Methods. 1998 Apr;71(2):177-86. doi: 10.1016/s0166-0934(97)00214-0. J Virol Methods. 1998. PMID: 9626951
-
Flow cytometric determination of ganciclovir susceptibilities of human cytomegalovirus clinical isolates.J Clin Microbiol. 1998 Apr;36(4):958-64. doi: 10.1128/JCM.36.4.958-964.1998. J Clin Microbiol. 1998. PMID: 9542916 Free PMC article.
-
Oligonucleotides as antivirals: dream or realistic perspective?Antiviral Res. 2006 Sep;71(2-3):307-16. doi: 10.1016/j.antiviral.2006.03.003. Epub 2006 Apr 3. Antiviral Res. 2006. PMID: 16621039 Review.
Cited by
-
Phenotypic drug susceptibility assay for influenza virus neuraminidase inhibitors.Clin Diagn Lab Immunol. 2004 Jan;11(1):21-8. doi: 10.1128/cdli.11.1.21-28.2004. Clin Diagn Lab Immunol. 2004. PMID: 14715540 Free PMC article.
-
Inhibition of ganciclovir-susceptible and -resistant human cytomegalovirus clinical isolates by the benzimidazole L-riboside 1263W94.Clin Diagn Lab Immunol. 2001 Nov;8(6):1279-81. doi: 10.1128/CDLI.8.6.1279-1281.2001. Clin Diagn Lab Immunol. 2001. PMID: 11687477 Free PMC article.
-
Susceptibilities of human cytomegalovirus clinical isolates to BAY38-4766, BAY43-9695, and ganciclovir.Antimicrob Agents Chemother. 2001 Oct;45(10):2925-7. doi: 10.1128/AAC.45.10.2925-2927.2001. Antimicrob Agents Chemother. 2001. PMID: 11557492 Free PMC article.
-
Applications of flow cytometry to clinical microbiology.Clin Microbiol Rev. 2000 Apr;13(2):167-95. doi: 10.1128/CMR.13.2.167. Clin Microbiol Rev. 2000. PMID: 10755996 Free PMC article. Review.
-
Use of a single monoclonal antibody to determine the susceptibilities of herpes simplex virus type 1 and type 2 clinical isolates to acyclovir.Clin Diagn Lab Immunol. 2002 Nov;9(6):1379-81. doi: 10.1128/cdli.9.6.1379-1381.2002. Clin Diagn Lab Immunol. 2002. PMID: 12414779 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
