Review
FASAY: a simple functional assay in yeast for identification of p53 mutation in tumors
Affiliations
- PMID: 10466430
Item in Clipboard
Review
FASAY: a simple functional assay in yeast for identification of p53 mutation in tumors
Neoplasma.
1999.
Abstract
Alteration of the p53 tumor suppressor gene is the most common genetic defect known to occur in human tumors. Germ-line p53 mutations significantly increase the risk of developing diverse malignancies. FASAY is a simple functional assay for germ-line and somatic mutations in the p53 gene altering the transactivation capability of the p53 protein. The method was successfully used for mutation analysis of p53 in various cell lines, somatic tumor cells and blood cells. In addition, FASAY was also found effective as a tool for basic research of binding of mutant p53 proteins to promoters of different p53 target genes.
Similar articles
-
[Functional screening of p53 status in tumor cells using Saccharomyces cerevisiae].Gan To Kagaku Ryoho. 1997 Feb;24(4):466-70. Gan To Kagaku Ryoho. 1997. PMID: 9063485 Review. Japanese.
-
Analysis of p53 status in human cell lines using a functional assay in yeast: detection of new non-sense p53 mutation in codon 124.Oncol Rep. 2005 Oct;14(4):901-7. Oncol Rep. 2005. PMID: 16142349
-
Screening patients for heterozygous p53 mutations using a functional assay in yeast.Nat Genet. 1993 Oct;5(2):124-9. doi: 10.1038/ng1093-124. Nat Genet. 1993. PMID: 8252037
-
Screen p53 mutations in hepatocellular carcinoma by FASAY: a novel splicing mutation.J Zhejiang Univ Sci B. 2007 Feb;8(2):81-7. doi: 10.1631/jzus.2007.B0081. J Zhejiang Univ Sci B. 2007. PMID: 17266182 Free PMC article.
-
Functional analysis of p53 tumor suppressor in yeast.Differentiation. 2005 Jul;73(6):261-77. doi: 10.1111/j.1432-0436.2005.00028.x. Differentiation. 2005. PMID: 16138827 Review.
Cited by
-
Humanized yeast to model human biology, disease and evolution.Dis Model Mech. 2022 Jun 1;15(6):dmm049309. doi: 10.1242/dmm.049309. Epub 2022 Jun 6. Dis Model Mech. 2022. PMID: 35661208 Free PMC article. Review.
-
Opposite regulation of MDM2 and MDMX expression in acquisition of mesenchymal phenotype in benign and cancer cells.Oncotarget. 2015 Nov 3;6(34):36156-71. doi: 10.18632/oncotarget.5392. Oncotarget. 2015. PMID: 26416355 Free PMC article.
-
p53, p63 and p73 in the wonderland of S. cerevisiae.Oncotarget. 2017 Jun 16;8(34):57855-57869. doi: 10.18632/oncotarget.18506. eCollection 2017 Aug 22. Oncotarget. 2017. PMID: 28915717 Free PMC article. Review.
-
The Diverse Functions of Mutant 53, Its Family Members and Isoforms in Cancer.Int J Mol Sci. 2019 Dec 7;20(24):6188. doi: 10.3390/ijms20246188. Int J Mol Sci. 2019. PMID: 31817935 Free PMC article. Review.
-
From regulation to deregulation of p53 in hematologic malignancies: implications for diagnosis, prognosis and therapy.Biomark Res. 2024 Nov 14;12(1):137. doi: 10.1186/s40364-024-00676-9. Biomark Res. 2024. PMID: 39538363 Free PMC article. Review.
Publication types
MeSH terms
LinkOut - more resources
Research Materials
Miscellaneous