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. 1999 Aug;11(8):1499-508.
doi: 10.1105/tpc.11.8.1499.

Identification and characterization of a prevacuolar compartment in stigmas of nicotiana alata

EA Miller  1 , MC Lee, MA Anderson
Affiliations

Identification and characterization of a prevacuolar compartment in stigmas of nicotiana alata

EA Miller et al. Plant Cell. 1999 Aug.

Abstract

The stigmas of the ornamental tobacco plant Nicotiana alata accumulate large quantities of a series of 6-kD proteinase inhibitors (PIs) in the central vacuole that are derived from a 40-kD precursor protein, Na-PI. The sorting information that directs Na-PI to the vacuole is likely to reside in a C-terminal propeptide domain of 25 amino acids that forms an amphipathic alpha helix. Using cell fractionation techniques, we have examined transit of Na-PI through the endomembrane system and have identified a prevacuolar compartment that contains Na-PI with an intact targeting signal. In contrast, the targeting signal is not present on the predominant form of Na-PI in the vacuole. The prevacuolar compartment is marked by the presence of homologs of both the t-SNARE, PEP12p, and the putative vacuolar sorting receptor BP-80. Cross-linking and affinity precipitation studies revealed that Na-PI associates with BP-80 within this compartment, providing in vivo evidence for the function of BP-80 as a sorting receptor for a protein with a C-terminal vacuolar targeting signal.

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References

    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. Nat Struct Biol. 1999 Jun;6(6):526-30 - PubMed
    1. Plant Physiol. 1997 May;114(1):325-36 - PubMed
    1. J Cell Biol. 1998 Oct 5;143(1):1-4 - PubMed
    1. Plant Physiol. 1996 Jun;111(2):469-74 - PubMed