Requirement for Wnt3 in vertebrate axis formation
- PMID: 10431240
- DOI: 10.1038/11932
Requirement for Wnt3 in vertebrate axis formation
Abstract
Several studies have implicated Wnt signalling in primary axis formation during vertebrate embryogenesis, yet no Wnt protein has been shown to be essential for this process. In the mouse, primitive streak formation is the first overt morphological sign of the anterior-posterior axis. Here we show that Wnt3 is expressed before gastrulation in the proximal epiblast of the egg cylinder, then is restricted to the posterior proximal epiblast and its associated visceral endoderm and subsequently to the primitive streak and mesoderm. Wnt3-/- mice develop a normal egg cylinder but do not form a primitive streak, mesoderm or node. The epiblast continues to proliferate in an undifferentiated state that lacks anterior-posterior neural patterning, but anterior visceral endoderm markers are expressed and correctly positioned. Our results suggest that regional patterning of the visceral endoderm is independent of primitive streak formation, but the subsequent establishment of anterior-posterior neural pattern in the ectoderm is dependent on derivatives of the primitive streak. These studies provide genetic proof for the requirement of Wnt3 in primary axis formation in the mouse.
Similar articles
-
Primitive streak formation in mice is preceded by localized activation of Brachyury and Wnt3.Dev Biol. 2005 Dec 15;288(2):363-71. doi: 10.1016/j.ydbio.2005.09.012. Epub 2005 Nov 10. Dev Biol. 2005. PMID: 16289026
-
Wnt3 signaling in the epiblast is required for proper orientation of the anteroposterior axis.Dev Biol. 2007 Dec 1;312(1):312-20. doi: 10.1016/j.ydbio.2007.09.030. Epub 2007 Sep 26. Dev Biol. 2007. PMID: 18028899
-
Beta-catenin signaling marks the prospective site of primitive streak formation in the mouse embryo.Dev Dyn. 2004 Oct;231(2):416-24. doi: 10.1002/dvdy.20135. Dev Dyn. 2004. PMID: 15366019
-
Building the mouse gastrula: signals, asymmetry and lineages.Curr Opin Genet Dev. 2006 Aug;16(4):419-25. doi: 10.1016/j.gde.2006.06.008. Epub 2006 Jun 21. Curr Opin Genet Dev. 2006. PMID: 16793258 Review.
-
Early patterning of the mouse embryo: implications for hematopoietic commitment and differentiation.Exp Hematol. 2005 Sep;33(9):1015-20. doi: 10.1016/j.exphem.2005.06.001. Exp Hematol. 2005. PMID: 16140149 Review.
Cited by
-
Myosin phosphatase modulates the cardiac cell fate by regulating the subcellular localization of Nkx2.5 in a Wnt/Rho-associated protein kinase-dependent pathway.Circ Res. 2013 Jan 18;112(2):257-66. doi: 10.1161/CIRCRESAHA.112.275818. Epub 2012 Nov 20. Circ Res. 2013. PMID: 23168335 Free PMC article.
-
Vertebrate cranial mesoderm: developmental trajectory and evolutionary origin.Cell Mol Life Sci. 2020 May;77(10):1933-1945. doi: 10.1007/s00018-019-03373-1. Epub 2019 Nov 13. Cell Mol Life Sci. 2020. PMID: 31722070 Free PMC article. Review.
-
Hypoblast controls mesoderm generation and axial patterning in the gastrulating rabbit embryo.Dev Genes Evol. 2004 Dec;214(12):591-605. doi: 10.1007/s00427-004-0436-y. Epub 2004 Oct 6. Dev Genes Evol. 2004. PMID: 15480760
-
Inhibition of the Wnt signaling pathway by Idax, a novel Dvl-binding protein.Mol Cell Biol. 2001 Jan;21(1):330-42. doi: 10.1128/MCB.21.1.330-342.2001. Mol Cell Biol. 2001. PMID: 11113207 Free PMC article.
-
Specifying the Anterior Primitive Streak by Modulating YAP1 Levels in Human Pluripotent Stem Cells.Stem Cell Reports. 2018 Dec 11;11(6):1357-1364. doi: 10.1016/j.stemcr.2018.10.013. Epub 2018 Nov 15. Stem Cell Reports. 2018. PMID: 30449705 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Molecular Biology Databases
