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. 1999 Jul 9;98(1):69-80.
doi: 10.1016/S0092-8674(00)80607-8.

Endomembrane trafficking of ras: the CAAX motif targets proteins to the ER and Golgi

E Choy  1 , V K Chiu, J Silletti, M Feoktistov, T Morimoto, D Michaelson, I E Ivanov, M R Philips
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Free article

Endomembrane trafficking of ras: the CAAX motif targets proteins to the ER and Golgi

E Choy et al. Cell. .
Free article

Abstract

We show that Nras is transiently localized in the Golgi prior to the plasma membrane (PM). Moreover, green fluorescent protein (GFP)-tagged Nras illuminated motile, peri-Golgi vesicles, and prolonged BFA treatment blocked PM expression. GFP-Hras colocalized with GFP-Nras, but GFP-Kras4B revealed less Golgi and no vesicular fluorescence. Whereas a secondary membrane targeting signal was required for PM expression, the CAAX motif alone was necessary and sufficient to target proteins to the endomembrane where they were methylated, a modification required for efficient membrane association. Thus, prenylated CAAX proteins do not associate directly with the PM but instead associate with the endomembrane and are subsequently transported to the PM, a process that requires a secondary targeting motif.

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