Spermatogonial stem cell transplantation, cryopreservation and culture
- PMID: 10366284
- DOI: 10.1006/scdb.1998.0205
Spermatogonial stem cell transplantation, cryopreservation and culture
Abstract
Testis cells of a fertile male mouse can be transplanted to the seminiferous tubules of an infertile male, where the donor spermatogonial stem cells will establish spermatogenesis and produce spermatozoa that transmit the donor haplotype to progeny. In addition, stem cells can be cryopreserved for long periods, thereby making male germ lines immortal. Recently, mouse testis cells have been cultured for longer than 3 months and, following transplantation, produced spermatogenesis. These techniques are likely to be applicable to many species, since rat testis cells can be cryopreserved and generate spermatogenesis in the seminiferous tubules of immunodeficient mice.
Comment in
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Introduction: the male germ cell; origin, migration, proliferation and differentiation.Semin Cell Dev Biol. 1998 Aug;9(4):379-91. doi: 10.1006/scdb.1998.0254. Semin Cell Dev Biol. 1998. PMID: 9813185 No abstract available.
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