Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1976 Dec;58(6):1419-27.
doi: 10.1172/JCI108597.

Translation and stability of human globin mRNA in Xenopus oocytes

G M Maniatis, F Ramirez, A Cann, P A Marks, A Bank

Translation and stability of human globin mRNA in Xenopus oocytes

G M Maniatis et al. J Clin Invest. 1976 Dec.

Abstract

Human globin messenger RNA (mRNA) prepared from erythroid cells of patients with sickle cell anemia has been translated in Xenopus laevis oocytes. Addition of hemin to the injected mRNA causes total globin synthesis to increase and the ratio of alpha- to betas-globin synthesis (alpha/betas ratio) to approach unity. To determine the effect of the length of the poly-(A) segment on human globin mRNA stability, 10 S globin mRNA was fractionated into poly-(A)-poor fractions by oligo (dT)-cellulose column chromatography. When oocytes are injected with each of these fractions, translation of the poly-(A)-rich globin mRNA is sustained for a longer period than that of the poly-(A)-poor mRNA. Regardless of the mRNA fraction injected, the alpha/betas ratio of the synthesized globin decreases as the injected oocytes are incubated for longer periods. The results indicate that in frog oocytes poly-(A)-rich mRNA has greater translational stability than poly-(A)-poor mRNA, AND beta-mRNA has greater stability than alpha-mRNA with comparable poly-(A) content.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Biochim Biophys Acta. 1958 Dec;30(3):608-15 - PubMed
    1. Proc Natl Acad Sci U S A. 1972 May;69(5):1299-303 - PubMed
    1. J Mol Biol. 1972 Jan 28;63(2):247-64 - PubMed
    1. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1809-13 - PubMed
    1. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1022-6 - PubMed

Publication types