A deuterium nuclear magnetic resonance study of the condensing effect of cholesterol on egg phosphatidylcholine bilayer membranes. I. Perdeuterated fatty acid probes
- PMID: 1033045
- DOI: 10.1016/0009-3084(76)90070-0
A deuterium nuclear magnetic resonance study of the condensing effect of cholesterol on egg phosphatidylcholine bilayer membranes. I. Perdeuterated fatty acid probes
Abstract
Deuterium nuclear magnetic resonance quadrupole splittings Dq and the related acyl chain segmental order parameters Smol have been determined for perdeuteriostearic acid intercalated as a molecular probe in the lamellar liquid crystalline phase of egg yolk phosphatidylcholine-cholesterol-water mixtures. The 2H NMR data show that cholesterol induces a high degree of order in the acyl chains of the phospholipid while maintaining the general profile of high order near the head group and relatively low order in the middle of the bilayer. This results in a pronounced thickening of the bilayer and concomitant decrease in the average molecular area of the fatty acyl chains. The giometrical changes in the bilayer due to cholesterol are discussed in terms of trans-gauche isomerization in the fatty acyl chains. The picture of the condensing effect revealed by 2H NMR is consistent with that from previous X-ray diffraction experiments. No evidence for a specific complex between cholesterol and phosphatidylcholine is apparent. The condensing effect of cholesterol is attributed to interaction between the fatty acyl chains and the rigid steroid nucleus, and to solvation of the 3beta-hydroxyl group at the aqueous interface.
Similar articles
-
Molecular motion and order in single-bilayer vesicles and multilamellar dispersions of egg lecithin and lecithin-cholesterol mixtures. A deuterium nuclear magnetic resonance study of specifically labeled lipids.Biochemistry. 1976 Mar 9;15(5):954-66. doi: 10.1021/bi00650a003. Biochemistry. 1976. PMID: 943179
-
A structural model for the cholesterol-phosphatidylcholine complexes in bilayer membranes.Lipids. 1977 Apr;12(4):348-56. doi: 10.1007/BF02533637. Lipids. 1977. PMID: 558491
-
Deuterium magnetic resonance of selectively deuterated cholesteryl esters in phosphatidylcholine vesicles.Biochemistry. 1980 Jul 22;19(15):3422-9. doi: 10.1021/bi00556a003. Biochemistry. 1980. PMID: 7190833
-
Deuterium magnetic resonance: theory and application to lipid membranes.Q Rev Biophys. 1977 Aug;10(3):353-418. doi: 10.1017/s0033583500002948. Q Rev Biophys. 1977. PMID: 335428 Review. No abstract available.
-
Cholesterol's Condensing Effect: Unpacking a Century-Old Mystery.JACS Au. 2021 Dec 17;2(1):84-91. doi: 10.1021/jacsau.1c00493. eCollection 2022 Jan 24. JACS Au. 2021. PMID: 35098224 Free PMC article. Review.
Cited by
-
Determination of the orientation and dynamics of ergosterol in model membranes using uniform 13C labeling and dynamically averaged 13C chemical shift anisotropies as experimental restraints.Biophys J. 2005 Aug;89(2):1120-31. doi: 10.1529/biophysj.105.059857. Epub 2005 May 27. Biophys J. 2005. PMID: 15923221 Free PMC article.
-
Ternary phase diagram of dipalmitoyl-PC/dilauroyl-PC/cholesterol: nanoscopic domain formation driven by cholesterol.Biophys J. 2001 Jun;80(6):2775-88. doi: 10.1016/S0006-3495(01)76245-5. Biophys J. 2001. PMID: 11371452 Free PMC article.
-
Order parameters and areas in fluid-phase oriented lipid membranes using wide angle X-ray scattering.Biophys J. 2008 Jul;95(2):669-81. doi: 10.1529/biophysj.107.127845. Epub 2008 Apr 4. Biophys J. 2008. PMID: 18390624 Free PMC article.
-
Behavior of cholesterol and its effect on head group and chain conformations in lipid bilayers: a molecular dynamics study.Biophys J. 1995 Jan;68(1):164-70. doi: 10.1016/S0006-3495(95)80171-2. Biophys J. 1995. PMID: 7711238 Free PMC article.
-
Glycosphingolipid fatty acid arrangement in phospholipid bilayers: cholesterol effects.Biophys J. 1995 Jan;68(1):179-86. doi: 10.1016/S0006-3495(95)80173-6. Biophys J. 1995. PMID: 7711240 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
