Poly(ADP-ribosyl)ation of p53 during apoptosis in human osteosarcoma cells
- PMID: 10232607
Poly(ADP-ribosyl)ation of p53 during apoptosis in human osteosarcoma cells
Abstract
Spontaneous apoptosis in human osteosarcoma cells was observed to be associated with a marked increase in the intracellular abundance of p53. Immunoprecipitation and immunoblot analysis revealed that, together with a variety of other nuclear proteins, p53 undergoes extensive poly(ADP-ribosyl)ation early during the apoptotic program in these cells. Subsequent degradation of poly(ADP-ribose) (PAR), attached to p53 presumably by PAR glycohydrolase, the only reported enzyme to degrade PAR, was apparent concomitant with the onset of proteolytic processing and activation of caspase-3, caspase-3-mediated cleavage of poly(ADP-ribose) polymerase (PARP), and internucleosomal DNA fragmentation during the later stages of cell death. The decrease in PAR covalently bound to p53 also coincided with the marked induction of expression of the p53-responsive genes bax and Fas. These results suggest that poly(ADP-ribosyl)ation may play a role in the regulation of p53 function and implies a regulatory role for PARP and/or PAR early in apoptosis.
Similar articles
-
Intact cell evidence for the early synthesis, and subsequent late apopain-mediated suppression, of poly(ADP-ribose) during apoptosis.Exp Cell Res. 1997 May 1;232(2):313-21. doi: 10.1006/excr.1997.3536. Exp Cell Res. 1997. PMID: 9168807
-
Poly(ADP-ribosyl)ation of p53 in vitro and in vivo modulates binding to its DNA consensus sequence.Neoplasia. 2001 May-Jun;3(3):179-88. doi: 10.1038/sj.neo.7900155. Neoplasia. 2001. PMID: 11494111 Free PMC article.
-
Characterization of distinct consecutive phases in non-genotoxic p53-induced apoptosis of Ewing tumor cells and the rate-limiting role of caspase 8.Oncogene. 2000 Aug 24;19(36):4096-107. doi: 10.1038/sj.onc.1203780. Oncogene. 2000. PMID: 10962570
-
Importance of poly(ADP-ribose) glycohydrolase in the control of poly(ADP-ribose) metabolism.Exp Cell Res. 2001 Aug 1;268(1):7-13. doi: 10.1006/excr.2001.5263. Exp Cell Res. 2001. PMID: 11461113 Review.
-
Poly(ADP-ribosyl)ation regulation of life and death in the nervous system.Cell Mol Life Sci. 2005 Apr;62(7-8):760-8. doi: 10.1007/s00018-004-4508-y. Cell Mol Life Sci. 2005. PMID: 15868401 Review.
Cited by
-
Protection by antioxidants against toxicity and apoptosis induced by the sulphur mustard analog 2-chloroethylethyl sulphide (CEES) in Jurkat T cells and normal human lymphocytes.Br J Pharmacol. 2004 Mar;141(5):795-802. doi: 10.1038/sj.bjp.0705591. Epub 2004 Feb 9. Br J Pharmacol. 2004. PMID: 14769780 Free PMC article.
-
DNA excision repair and DNA damage-induced apoptosis are linked to Poly(ADP-ribosyl)ation but have different requirements for p53.Mol Cell Biol. 2000 Sep;20(18):6695-703. doi: 10.1128/MCB.20.18.6695-6703.2000. Mol Cell Biol. 2000. PMID: 10958667 Free PMC article.
-
Inhibition of poly(ADP-ribose) polymerase activity is insufficient to induce tetraploidy.Nucleic Acids Res. 2001 Feb 1;29(3):841-9. doi: 10.1093/nar/29.3.841. Nucleic Acids Res. 2001. PMID: 11160908 Free PMC article.
-
Glucose stimulates protein modification by O-linked GlcNAc in pancreatic beta cells: linkage of O-linked GlcNAc to beta cell death.Proc Natl Acad Sci U S A. 2000 Mar 14;97(6):2820-5. doi: 10.1073/pnas.97.6.2820. Proc Natl Acad Sci U S A. 2000. PMID: 10717000 Free PMC article.
-
Retracted Article: Overexpression of PCDH8 inhibits proliferation and invasion, and induces apoptosis in papillary thyroid cancer cells.RSC Adv. 2018 May 16;8(32):18030-18037. doi: 10.1039/c8ra02291g. eCollection 2018 May 14. RSC Adv. 2018. Retraction in: RSC Adv. 2021 Jan 20;11(7):4163. doi: 10.1039/d1ra90011k PMID: 35542088 Free PMC article. Retracted.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Other Literature Sources
Medical
Research Materials
Miscellaneous