Abstract
Antibodies directed against membrane components of dog pancreas rough endoplasmic reticulum (A-RER) and rat liver Golgi apparatus (A-Golgi) (Louvard, D., H. Reggio, and G. Warren, 1982, J. Cell Biol. 92:92-107) have been applied to cultured rat prolactin (PRL) cells, either normal cells in primary cultures, or clonal GH3 cells. In normal PRL cells, the A-RER stained the membranes of the perinuclear cisternae as well as those of many parallel RER cisternae. The A-Golgi stained part of the Golgi membranes. In the stacks it stained the medial saccules and, with a decreasing intensity, the saccules of the trans side, as well as, in some cells, a linear cisterna in the center of the Golgi zone. It also stained the membrane of many small vesicles as well as that of lysosomelike structures in all cells. In contrast, it never stained the secretory granule membrane, except at the level of very few segregating granules on the trans face of the Golgi zone. In GH3 cells the A-RER stained the membrane of the perinuclear cisternae, as well as that of short discontinuous flat cisternae. The A-Golgi stained the same components of the Golgi zone as in normal PRL cells. In some cells of both types the A-Golgi also stained discontinuous patches on the plasma membrane and small vesicles fusing with the plasma membrane. Immunostaining of Golgi membranes revealed modifications of membrane flow in relation to either acute stimulation of PRL release by thyroliberin or inhibition of basal secretion by monensin.
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Selected References
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- Bergeron J. J. Golgi fractions from livers of control and ethanol-intoxicated rats. Enzymic and morphologic properties following rapid isolation. Biochim Biophys Acta. 1979 Aug 23;555(3):493–503. doi: 10.1016/0005-2736(79)90402-4. [DOI] [PubMed] [Google Scholar]
- Brinkley B. R., Murphy P., Richardson L. C. Procedure for embedding in situ selected cells cultured in vitro. J Cell Biol. 1967 Oct;35(1):279–283. doi: 10.1083/jcb.35.1.279. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Drouin J., Lagacé L., Labrie F. Estradiol-induced increase of the LH responsive to LH releasing hormone (LHRH) in rat anterior pituitary cells in culture. Endocrinology. 1976 Dec;99(6):1477–1481. doi: 10.1210/endo-99-6-1477. [DOI] [PubMed] [Google Scholar]
- Farquhar M. G. Membrane recycling in secretory cells: implications for traffic of products and specialized membranes within the Golgi complex. Methods Cell Biol. 1981;23:399–427. doi: 10.1016/s0091-679x(08)61511-3. [DOI] [PubMed] [Google Scholar]
- Farquhar M. G., Palade G. E. The Golgi apparatus (complex)-(1954-1981)-from artifact to center stage. J Cell Biol. 1981 Dec;91(3 Pt 2):77s–103s. doi: 10.1083/jcb.91.3.77s. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Farquhar M. G., Reid J. J., Daniell L. W. Intracellular transport and packaging of prolactin: a quantitative electron microscope autoradiographic study of mammotrophs dissociated from rat pituitaries. Endocrinology. 1978 Jan;102(1):296–311. doi: 10.1210/endo-102-1-296. [DOI] [PubMed] [Google Scholar]
- Gourdji D., Kerdelhue B., Tixier-Vidal A. Ultrastruture d'un clone de cellules hypophysaires sécrétant de la prolactine (clone GH3). Modifications induites par l'hormone hypothalamique de libération de l'hormone thyréotrope (TRF. C R Acad Sci Hebd Seances Acad Sci D. 1972 Jan 17;274(3):437–440. [PubMed] [Google Scholar]
- Graham R. C., Jr, Karnovsky M. J. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem. 1966 Apr;14(4):291–302. doi: 10.1177/14.4.291. [DOI] [PubMed] [Google Scholar]
- Louvard D., Reggio H., Warren G. Antibodies to the Golgi complex and the rough endoplasmic reticulum. J Cell Biol. 1982 Jan;92(1):92–107. doi: 10.1083/jcb.92.1.92. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Novikoff A. B., Novikoff P. M. Cytochemical contributions to differentiating GERL from the Golgi apparatus. Histochem J. 1977 Sep;9(5):525–551. doi: 10.1007/BF01002901. [DOI] [PubMed] [Google Scholar]
- PASTEELS J. L. RECHERCHES MORPHOLOGIQUES ET EXP'ERIMENTALES SUR LA S'ECR'ETION DE PROLACTINE. Arch Biol (Liege) 1963;74:439–553. [PubMed] [Google Scholar]
- Palade G. Intracellular aspects of the process of protein synthesis. Science. 1975 Aug 1;189(4200):347–358. doi: 10.1126/science.1096303. [DOI] [PubMed] [Google Scholar]
- Racadot J., Olivier L., Porcile E., Droz B. Appareil de Golgi et origine des grains de sécrétion dans les cellules adénohypophysaires chez le rat. Etude radioautographique en microscopie électronique après injection de leucine tritiée. C R Acad Sci Hebd Seances Acad Sci D. 1965 Oct 11;261(15):2972–2974. [PubMed] [Google Scholar]
- Tartakoff A., Vassalli P., Détraz M. Comparative studies of intracellular transport of secretory proteins. J Cell Biol. 1978 Dec;79(3):694–707. doi: 10.1083/jcb.79.3.694. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tixier-Vidal A., Gourdji D., Tougard C. A cell culture approach to the study of anterior pituitary cells. Int Rev Cytol. 1975;41:173–239. doi: 10.1016/s0074-7696(08)60968-7. [DOI] [PubMed] [Google Scholar]
- Tixier-Vidal A., Picart R. Etude quantitative par radioautographie au microscope electronique de l'utilisation de la DL-leucine-3H par les cellules de l'hypophyse du canard en culture organotypiqe. J Cell Biol. 1967 Dec;35(3):501–519. doi: 10.1083/jcb.35.3.501. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tougard C., Morin A., Picart R., Tixier-Vidal A. Rôle du réticulum endoplasmique dans la sécrétion de prolactine. Ann Endocrinol (Paris) 1981 Oct-Nov;42(4-5):342–348. [PubMed] [Google Scholar]
- Tougard C., Picart R., Tixier-Vidal A. Electron-microscopic cytochemical studies on the secretory process in rat prolactin cells in primary culture. Am J Anat. 1980 Aug;158(4):471–490. doi: 10.1002/aja.1001580409. [DOI] [PubMed] [Google Scholar]