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. 1984 Mar;49(3):701–708. doi: 10.1128/jvi.49.3.701-708.1984

Antigenic analysis of woodchuck hepatitis virus surface antigen with site-specific radioimmunoassays.

P J Cote, R E Engle, C A Langer, A Ponzetto, J L Gerin
PMCID: PMC255527  PMID: 6199511

Abstract

Monoclonal antibodies to five nonoverlapping antigenic domains of woodchuck hepatitis virus surface antigen (WHsAg) were used to develop site-specific radioimmunoassays. The assays were based on the solid-phase sandwich principle in which different combinations of individual domain-specific antibodies were used as immunoadsorbents and radioiodinated probes. Over 85% of the combinations tested were able to detect serum WHsAg, including those using the same antibody as immunoadsorbent and probe. The limits for antigen detection in one site-specific system ranged between 16 and 80 ng of WHsAg per ml. The antigenic similarity of serum WHsAg from 13 colony woodchucks was shown with several combination assay systems. WHsAg was equally immunoreactive in these assay systems whether obtained by immunoaffinity chromatography or standard rate zone centrifugation methods. Further site-specific analysis demonstrated that Formalin treatment of purified antigen did not affect the immunoreactivity of these WHsAg sites.

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Selected References

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  1. Budkowska A., Shih J. W., Gerin J. L. Immunochemistry and polypeptide composition of hepatitis B core antigen (HBc Ag). J Immunol. 1977 Apr;118(4):1300–1305. [PubMed] [Google Scholar]
  2. Cote P. J., Jr, Dapolito G. M., Shih J. W., Gerin J. L. Surface antigenic determinants of mammalian "hepadnaviruses" defined by group- and class-specific monoclonal antibodies. J Virol. 1982 Apr;42(1):135–142. doi: 10.1128/jvi.42.1.135-142.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cote P. J., Jr, Gerin J. L. Nonoverlapping antigenic sites of woodchuck hepatitis virus surface antigen and their cross-reactivity with ground squirrel hepatitis virus and hepatitis B virus surface antigens. J Virol. 1983 Jul;47(1):15–23. doi: 10.1128/jvi.47.1.15-23.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gerin J. L., Faust R. M., Holland P. V. Biophysical characterization of the adr subtype of hepatitis B antigen and preparation of anti-r sera in rabbits. J Immunol. 1975 Jul;115(1):100–105. [PubMed] [Google Scholar]
  5. Gerin J. L., Ford E. C., Purcell R. H. Biochemical characterization of Australia antigen. Evidence for defective particles of hepatitis B virus. Am J Pathol. 1975 Dec;81(3):651–668. [PMC free article] [PubMed] [Google Scholar]
  6. Goodall A. H., Meek F. L., Waters J. A., Miescher G. C., Janossy G., Thomas H. C. A rapid one-step radiometric assay for hepatitis B surface antigen utilizing monoclonal antibodies. J Immunol Methods. 1982 Jul 30;52(2):167–174. doi: 10.1016/0022-1759(82)90042-4. [DOI] [PubMed] [Google Scholar]
  7. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  8. Kaplan P. M., Greenman R. L., Gerin J. L., Purcell R. H., Robinson W. S. DNA polymerase associated with human hepatitis B antigen. J Virol. 1973 Nov;12(5):995–1005. doi: 10.1128/jvi.12.5.995-1005.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Katus H. A., Hurrell J. G., Matsueda G. R., Ehrlich P., Zurawski V. R., Jr, Khaw B. A., Haber E. Increased specificity in human cardiac-myosin radioimmunoassay utilizing two monoclonal antibodies in a double sandwich assay. Mol Immunol. 1982 Mar;19(3):451–455. doi: 10.1016/0161-5890(82)90211-5. [DOI] [PubMed] [Google Scholar]
  10. Marion P. L., Oshiro L. S., Regnery D. C., Scullard G. H., Robinson W. S. A virus in Beechey ground squirrels that is related to hepatitis B virus of humans. Proc Natl Acad Sci U S A. 1980 May;77(5):2941–2945. doi: 10.1073/pnas.77.5.2941. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Popper H., Shih J. W., Gerin J. L., Wong D. C., Hoyer B. H., London W. T., Sly D. L., Purcell R. H. Woodchuck hepatitis and hepatocellular carcinoma: correlation of histologic with virologic observations. Hepatology. 1981 Mar-Apr;1(2):91–98. doi: 10.1002/hep.1840010202. [DOI] [PubMed] [Google Scholar]
  12. Purcell R. H., Gerin J. L., Almeida J. B., Holland P. V. Radioimmunoassay for the detection of the core of the Dane particle and antibody to it. Intervirology. 1974;2(4):231–243. doi: 10.1159/000149428. [DOI] [PubMed] [Google Scholar]
  13. Purcell R. H., Gerin J. L. Hepatitis B vaccines. On the threshold. Am J Clin Pathol. 1978 Jul;70(1 Suppl):159–169. [PubMed] [Google Scholar]
  14. Robinson W. S. Genetic variation among hepatitis B and related viruses. Ann N Y Acad Sci. 1980;354:371–378. doi: 10.1111/j.1749-6632.1980.tb27978.x. [DOI] [PubMed] [Google Scholar]
  15. Shih J. W., Cote P. J., Jr, Dapolito G. M., Gerin J. L. Production of monoclonal antibodies against hepatitis B surface antigen (HBsAg) by somatic cell hybrids. J Virol Methods. 1980;1(5):257–273. doi: 10.1016/0166-0934(80)90023-3. [DOI] [PubMed] [Google Scholar]
  16. Stenman U. H., Sutinen M. L., Selander R. K., Tontti K., Schröder J. Characterization of a monoclonal antibody to human alpha-fetoprotein and its use in affinity chromatography. J Immunol Methods. 1981;46(3):337–345. doi: 10.1016/0022-1759(81)90319-7. [DOI] [PubMed] [Google Scholar]
  17. Summers J. Three recently described animal virus models for human hepatitis B virus. Hepatology. 1981 Mar-Apr;1(2):179–183. doi: 10.1002/hep.1840010215. [DOI] [PubMed] [Google Scholar]
  18. Wands J. R., Carlson R. I., Schoemaker H., Isselbacher K. J., Zurawski V. R., Jr Immunodiagnosis of hepatitis B with high-affinity IgM monoclonal antibodies. Proc Natl Acad Sci U S A. 1981 Feb;78(2):1214–1218. doi: 10.1073/pnas.78.2.1214. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wands J. R., Marciniak R. A., Isselbacher K. J., Varghese M., Don G., Halliday J. W., Powell L. W. Demonstration of previously undetected hepatitis B viral determinants in an Australian Aboriginal population by monoclonal anti-hbs antibody radioimmunoassays. Lancet. 1982 May 1;1(8279):977–980. doi: 10.1016/s0140-6736(82)91988-2. [DOI] [PubMed] [Google Scholar]
  20. Wong D. C., Shih J. W., Purcell R. H., Gerin J. L., London W. T. Natural and experimental infection of woodchucks with woodchuck hepatitis virus, as measured by new, specific assays for woodchuck surface antigen and antibody. J Clin Microbiol. 1982 Mar;15(3):484–490. doi: 10.1128/jcm.15.3.484-490.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

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