Abstract
1 In rabbit polymorphonuclear leukocytes (PMNLs) apomorphine at 10-100 microM inhibits fMet-Leu-Phe and A23187-induced exocytosis, and the phorbol myristate acetate- and fMet-Leu-Phe-induced activation of the metabolic burst. The secretory response was not restored by washing the cells after pretreatment with apomorphine. 2 The inhibitory effect of apomorphine was not prevented by the dopamine receptor antagonists haloperidol and pimozide, nor did dopamine itself inhibit fMet-Leu-Phe-induced exocytosis. It therefore seems unlikely that effects are mediated via dopamine receptors. However, sulphydryl reagents reduced the inhibitory effect of apomorphine, suggesting that it may depend upon interaction with susceptible sulphydryl groups, the intactness of which is required for exocytosis and other functions of PMNLs.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Battistella A., Boarato E., Bruni A., Mietto L., Palatini P., Toffano G. Apomorphine-induced inhibition of histamine release in rat peritoneal mast cells. Br J Pharmacol. 1986 Jun;88(2):457–462. doi: 10.1111/j.1476-5381.1986.tb10224.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Becker E. L., Showell H. J. The ability of chemotactic factors to induce lysosomal enzyme release. II. The mechanism of release. J Immunol. 1974 Jun;112(6):2055–2062. [PubMed] [Google Scholar]
- Carr S. R., Fozard J. R. Inhibition of 5-hydroxytryptamine-evoked autonomic transmitter release by apomorphine. Eur J Pharmacol. 1982 Jul 16;81(3):469–477. doi: 10.1016/0014-2999(82)90112-1. [DOI] [PubMed] [Google Scholar]
- Di Chiara G., Corsini G. U., Mereu G. P., Tissari A., Gessa G. L. Self-inhibitory dopamine receptors: their role in the biochemical and behavioral effects of low doses of apomorphine. Adv Biochem Psychopharmacol. 1978;19:275–292. [PubMed] [Google Scholar]
- Elferink J. G., Deierkauf M. Inhibition of polymorphonuclear leukocyte functions by chlortetracycline. Biochem Pharmacol. 1984 Nov 15;33(22):3667–3673. doi: 10.1016/0006-2952(84)90155-2. [DOI] [PubMed] [Google Scholar]
- Elferink J. G. Measurement of the metabolic burst in human neutrophils: a comparison between cytochrome c and NBT reduction. Res Commun Chem Pathol Pharmacol. 1984 Feb;43(2):339–342. [PubMed] [Google Scholar]
- Elferink J. G., Riemersma J. C. Effects of sulfhydryl reagents on phagocytosis and exocytosis in rabbit polymorphonuclear leukocytes. Chem Biol Interact. 1980 May;30(2):139–149. doi: 10.1016/0009-2797(80)90121-0. [DOI] [PubMed] [Google Scholar]
- Fantone J. C., Ward P. A. Role of oxygen-derived free radicals and metabolites in leukocyte-dependent inflammatory reactions. Am J Pathol. 1982 Jun;107(3):395–418. [PMC free article] [PubMed] [Google Scholar]
- Giordano G. F., Lichtman M. A. The role of sulfhydryl groups in human neutrophil adhesion, movement and particle ingestion. J Cell Physiol. 1973 Dec;82(3):387–395. doi: 10.1002/jcp.1040820308. [DOI] [PubMed] [Google Scholar]
- Goldstein I. M., Lind S., Hoffstein S., Weissmann G. Influence of local anesthetics upon human polymorphonuclear leukocyte function in vitro. Reduction of lysosomal enzyme release and superoxide anion production. J Exp Med. 1977 Aug 1;146(2):483–494. doi: 10.1084/jem.146.2.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Joost H. G., Göke B., Hasselblatt A. Effects of pimozide and apomorphine on insulin secretion from the perfused rat pancreas. Arch Int Pharmacodyn Ther. 1983 May;263(1):155–163. [PubMed] [Google Scholar]
- Mietto L., Battistella A., Toffano G., Bruni A. Modulation of lysophosphatidylserine-dependent histamine release. Agents Actions. 1984 Apr;14(3-4):376–378. doi: 10.1007/BF01973832. [DOI] [PubMed] [Google Scholar]
- Oliver J. M., Albertini D. F., Berlin R. D. Effects of glutathione-oxidizing agents on microtubule assembly and microtubule-dependent surface properties of human neutrophils. J Cell Biol. 1976 Dec;71(3):921–932. doi: 10.1083/jcb.71.3.921. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Strehler B. L. Bioluminescence assay: principles and practice. Methods Biochem Anal. 1968;16:99–181. doi: 10.1002/9780470110348.ch2. [DOI] [PubMed] [Google Scholar]
- Weissmann G., Smolen J. E., Korchak H. M. Release of inflammatory mediators from stimulated neutrophils. N Engl J Med. 1980 Jul 3;303(1):27–34. doi: 10.1056/NEJM198007033030109. [DOI] [PubMed] [Google Scholar]
- Yamashita T. Effect of maleimide derivatives, sulfhydryl reagents, on stimulation of neutrophil superoxide anion generation with concanavalin A. FEBS Lett. 1983 Dec 12;164(2):267–271. doi: 10.1016/0014-5793(83)80299-3. [DOI] [PubMed] [Google Scholar]