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. 1995 May;108(1):47–57. doi: 10.1104/pp.108.1.47

Induction of Anthocyanin Accumulation by Cytokinins in Arabidopsis thaliana.

J Deikman 1, P E Hammer 1
PMCID: PMC157304  PMID: 12228453

Abstract

Arabidopsis thaliana plants treated with exogenous cytokinins accumulate anthocyanin pigments. We have characterized this response because it is potentially useful as a genetic marker for cytokinin responsiveness. Levels of mRNAs for four genes of the anthocyanin biosynthesis pathway, phenylalanine ammonia lyase 1 (PAL1), chalcone synthase (CHS), chalcone isomerase (CHI), and dihydroflavonol reductase (DFR) were shown to increase coordinately in response to benzyladenine (BA). However, nuclear run-on transcription experiments suggested that although CHS and DFR are controlled by BA at the transcriptional level, PAL1 and CHI are controlled by BA posttranscriptionally. CHS mRNA levels increased within 2 h of BA spray application, and peaked by 3 h. Levels of PAL1 mRNA did not increase within 6 h of BA spray. We also showed that PAL1, CHS, CHI, and DFR mRNA levels fluctuate during a 24-h period and appear to be controlled by a circadian clock. The relation between cytokinin regulation and light regulation of CHS gene transcription is discussed.

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Selected References

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  1. Bleecker A. B., Estelle M. A., Somerville C., Kende H. Insensitivity to Ethylene Conferred by a Dominant Mutation in Arabidopsis thaliana. Science. 1988 Aug 26;241(4869):1086–1089. doi: 10.1126/science.241.4869.1086. [DOI] [PubMed] [Google Scholar]
  2. Chang C., Kwok S. F., Bleecker A. B., Meyerowitz E. M. Arabidopsis ethylene-response gene ETR1: similarity of product to two-component regulators. Science. 1993 Oct 22;262(5133):539–544. doi: 10.1126/science.8211181. [DOI] [PubMed] [Google Scholar]
  3. Crowell D. N., Kadlecek A. T., John M. C., Amasino R. M. Cytokinin-induced mRNAs in cultured soybean cells. Proc Natl Acad Sci U S A. 1990 Nov;87(22):8815–8819. doi: 10.1073/pnas.87.22.8815. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dehio C., de Bruijn F. J. The early nodulin gene SrEnod2 from Sesbania rostrata is inducible by cytokinin. Plant J. 1992 Jan;2(1):117–128. doi: 10.1046/j.1365-313x.1992.t01-51-00999.x. [DOI] [PubMed] [Google Scholar]
  5. Dominov J. A., Stenzler L., Lee S., Schwarz J. J., Leisner S., Howell S. H. Cytokinins and auxins control the expression of a gene in Nicotiana plumbaginifolia cells by feedback regulation. Plant Cell. 1992 Apr;4(4):451–461. doi: 10.1105/tpc.4.4.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dong X., Mindrinos M., Davis K. R., Ausubel F. M. Induction of Arabidopsis defense genes by virulent and avirulent Pseudomonas syringae strains and by a cloned avirulence gene. Plant Cell. 1991 Jan;3(1):61–72. doi: 10.1105/tpc.3.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dooner H. K., Robbins T. P., Jorgensen R. A. Genetic and developmental control of anthocyanin biosynthesis. Annu Rev Genet. 1991;25:173–199. doi: 10.1146/annurev.ge.25.120191.001133. [DOI] [PubMed] [Google Scholar]
  8. Kende H. The cytokinins. Int Rev Cytol. 1971;31:301–338. doi: 10.1016/s0074-7696(08)60061-3. [DOI] [PubMed] [Google Scholar]
  9. Klein A. O., Hagen C. W. Anthocyanin production in detached petals of impatiens balsamina L. Plant Physiol. 1961 Jan;36(1):1–9. doi: 10.1104/pp.36.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kubasek W. L., Shirley B. W., McKillop A., Goodman H. M., Briggs W., Ausubel F. M. Regulation of Flavonoid Biosynthetic Genes in Germinating Arabidopsis Seedlings. Plant Cell. 1992 Oct;4(10):1229–1236. doi: 10.1105/tpc.4.10.1229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lloyd A. M., Walbot V., Davis R. W. Arabidopsis and Nicotiana anthocyanin production activated by maize regulators R and C1. Science. 1992 Dec 11;258(5089):1773–1775. doi: 10.1126/science.1465611. [DOI] [PubMed] [Google Scholar]
  12. Lu J. L., Ertl J. R., Chen C. M. Cytokinin enhancement of the light induction of nitrate reductase transcript levels in etiolated barley leaves. Plant Mol Biol. 1990 Apr;14(4):585–594. doi: 10.1007/BF00027504. [DOI] [PubMed] [Google Scholar]
  13. Martin C., Gerats T. Control of Pigment Biosynthesis Genes during Petal Development. Plant Cell. 1993 Oct;5(10):1253–1264. doi: 10.1105/tpc.5.10.1253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Memelink J., Hoge J. H., Schilperoort R. A. Cytokinin stress changes the developmental regulation of several defence-related genes in tobacco. EMBO J. 1987 Dec 1;6(12):3579–3583. doi: 10.1002/j.1460-2075.1987.tb02688.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mo Y., Nagel C., Taylor L. P. Biochemical complementation of chalcone synthase mutants defines a role for flavonols in functional pollen. Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):7213–7217. doi: 10.1073/pnas.89.15.7213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ohl S., Hedrick S. A., Chory J., Lamb C. J. Functional properties of a phenylalanine ammonia-lyase promoter from Arabidopsis. Plant Cell. 1990 Sep;2(9):837–848. doi: 10.1105/tpc.2.9.837. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rochester D. E., Winer J. A., Shah D. M. The structure and expression of maize genes encoding the major heat shock protein, hsp70. EMBO J. 1986 Mar;5(3):451–458. doi: 10.1002/j.1460-2075.1986.tb04233.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Shirley B. W., Hanley S., Goodman H. M. Effects of ionizing radiation on a plant genome: analysis of two Arabidopsis transparent testa mutations. Plant Cell. 1992 Mar;4(3):333–347. doi: 10.1105/tpc.4.3.333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stabel P., Eriksson T., Engström P. Changes in Protein Synthesis upon Cytokinin-Mediated Adventitious Bud Induction and during Seedling Development in Norway Spruce, Picea abies. Plant Physiol. 1990 Apr;92(4):1174–1183. doi: 10.1104/pp.92.4.1174. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Suttle J. C. Cytokinin-induced ethylene biosynthesis in nonsenescing cotton leaves. Plant Physiol. 1986 Dec;82(4):930–935. doi: 10.1104/pp.82.4.930. [DOI] [PMC free article] [PubMed] [Google Scholar]

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