Abstract
We compared an automated microparticle double-antigen sandwich enzyme immunoassay (EIA) for the IMx test system recently developed by Abbott with two established assays (the automated indirect Vidas IgG EIA and the double-antigen sandwich EIA from Murex/Wellcome) devised for the detection of human immunodeficiency virus type 1 (HIV-1) and HIV-2 antibodies. A total of 1,078 consecutive serum samples were tested prospectively with the three assays. In addition, we used retrospectively selected panels of serum samples with discrepant results in two different screening tests and with indeterminate or positive Western immunoblot (WB) results, as well as five commercially available HIV-1 seroconversion panels. The new assay showed excellent discriminatory characteristics for the separation of samples from HIV-1-positive and HIV-1-negative persons according to Centers for Disease Control and Prevention WB criteria. The sensitivities were 98.1, 92.9, and 96.1% for the new test and the two other assays, respectively, and the specificities were 99.7, 97.9, and 98.1%, respectively. With the seroconversion panels this new test was positive several days earlier than the two other assays; i.e., seroconversion was evident at the peak of p24 antigenemia and often several weeks before WB became positive by the most stringent criteria.
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- Azevedo-Pereira J. M., Lourenço M. H., Barin F., Cisterna R., Denis F., Moncharmont P., Grillo R., Santos-Ferreira M. O. Multicenter evaluation of a fully automated screening test, VIDAS HIV 1 + 2, for antibodies to human immunodeficiency virus types 1 and 2. J Clin Microbiol. 1994 Oct;32(10):2559–2563. doi: 10.1128/jcm.32.10.2559-2563.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Crofts N., Maskill W., Gust I. D. Evaluation of enzyme-linked immunosorbent assays: a method of data analysis. J Virol Methods. 1988 Oct;22(1):51–59. doi: 10.1016/0166-0934(88)90087-0. [DOI] [PubMed] [Google Scholar]
- Genesca J., Shih J. W., Jett B. W., Hewlett I. K., Epstein J. S., Alter H. J. What do western blot indeterminate patterns for human immunodeficiency virus mean in EIA-negative blood donors? Lancet. 1989 Oct 28;2(8670):1023–1025. doi: 10.1016/s0140-6736(89)91027-1. [DOI] [PubMed] [Google Scholar]
- Ledergerber B., von Overbeck J., Egger M., Lüthy R. The Swiss HIV Cohort Study: rationale, organization and selected baseline characteristics. Soz Praventivmed. 1994;39(6):387–394. doi: 10.1007/BF01299670. [DOI] [PubMed] [Google Scholar]
- Matter L. Labordiagnose der HIV-Infektion. Schweiz Med Wochenschr. 1991 Aug 24;121(34):1183–1186. [PubMed] [Google Scholar]
- Midthun K., Garrison L., Clements M. L., Farzadegan H., Fernie B., Quinn T. Frequency of indeterminate western blot tests in healthy adults at low risk for human immunodeficiency virus infection. The NIAID AIDS Vaccine Clinical Trails Network. J Infect Dis. 1990 Dec;162(6):1379–1382. doi: 10.1093/infdis/162.6.1379. [DOI] [PubMed] [Google Scholar]
- Mortimer P. P. The fallibility of HIV western blot. Lancet. 1991 Feb 2;337(8736):286–287. doi: 10.1016/0140-6736(91)90884-r. [DOI] [PubMed] [Google Scholar]
- Pyra H., Böni J., Schüpbach J. Ultrasensitive retrovirus detection by a reverse transcriptase assay based on product enhancement. Proc Natl Acad Sci U S A. 1994 Feb 15;91(4):1544–1548. doi: 10.1073/pnas.91.4.1544. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sato P. A., Maskill W. J., Tamashiro H., Heymann D. L. Strategies for laboratory HIV testing: an examination of alternative approaches not requiring Western blot. Bull World Health Organ. 1994;72(1):129–134. [PMC free article] [PubMed] [Google Scholar]
- Telenti A., Uehlinger D. E., Marchesi F., Germann D., Malinverni R., Matter L. Epstein-Barr virus infection in HIV-positive patients. Eur J Clin Microbiol Infect Dis. 1993 Aug;12(8):601–609. doi: 10.1007/BF01973638. [DOI] [PubMed] [Google Scholar]