Abstract
To determine the storage temperature needed for the preservation of standard sera for serodiagnostic tests, groups of sera were tested before and after storage at -70 degrees C and -20 degrees C for periods of up to five years. The temperature required depended on the nature of the test, but no definite advantage was found in storage at -70 degrees C. For complement fixation this temperature was inadequate and greatly inferior to liquid nitrogen. For immunofluorescence it had no apparent advantage over a temperature of -20 degrees C, and for some other tests it was no more than marginally superior.
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Selected References
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- Pegg D. E. Long-term preservation of cells and tissues: a review. J Clin Pathol. 1976 Apr;29(4):271–285. doi: 10.1136/jcp.29.4.271. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ridley D. S. Comparative evaluation of complement fixing antigens for schistosomiasis. Trans R Soc Trop Med Hyg. 1973;67(6):793–796. doi: 10.1016/0035-9203(73)90005-9. [DOI] [PubMed] [Google Scholar]
- Stamm W. P., Phillips E. A. A cellulose acetate membrane precipitin (CAP) test for amoebiasis. Trans R Soc Trop Med Hyg. 1977;71(6):490–492. doi: 10.1016/0035-9203(77)90141-9. [DOI] [PubMed] [Google Scholar]
- Tosswill J. H., Ridley D. S., Warhurst D. C. Counter immunoelectrophoresis as a rapid screening test for amoebic liver abscess. J Clin Pathol. 1980 Jan;33(1):33–35. doi: 10.1136/jcp.33.1.33. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Woodhead B. G. The preservation of standard sera for helminth complement fixation tests. Trans R Soc Trop Med Hyg. 1971;65(6):830–834. doi: 10.1016/0035-9203(71)90099-x. [DOI] [PubMed] [Google Scholar]