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. 1979 Dec;32(3):934–942. doi: 10.1128/jvi.32.3.934-942.1979

Viable deletion mutant of human papovavirus BK that induces insulinomas in hamsters.

S Watanabe, K Yoshiike, A Nozawa, Y Yuasa, S Uchida
PMCID: PMC525942  PMID: 229273

Abstract

A plaque morphology mutant (pm-522) of human papovavirus BK, which was rescued from a human papovavirus BK-induced hamster pineocytoma, was characterized and compared with a cloned wild-type virus (wt-501). Mutant pm-522 formed turbid plaques and grew more slowly than wt-501 in human embryonic kidney (HEK) cells. The immunofluorescence assay revealed that more HEK cells underwent abortive infection with pm-522 than with wt-501. Whereas wt-501 induced brain tumors and osteosarcomas, but no insulinomas, in hamsters, pm-522 induced brain tumors and insulinomas. The DNA of pm-522 was found by electrophoresis and electron microscopy to have a deletion (85 +/- 15 base pairs) and an insertion (40 +/- 10 base pairs) between map coordinates 0.708 and 0.725 from the endonuclease EcoRI cleavage site. These results demonstrate the presence of a viable deletion human papovarivus BK mutant capable of inducing insulinomas in hamsters.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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