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. 1995 Aug 15;92(17):7672–7676. doi: 10.1073/pnas.92.17.7672

Identification of a plant serine-arginine-rich protein similar to the mammalian splicing factor SF2/ASF.

G Lazar 1, T Schaal 1, T Maniatis 1, H M Goodman 1
PMCID: PMC41207  PMID: 7644475

Abstract

We show that the higher plant Arabidopsis thaliana has a serine-arginine-rich (SR) protein family whose members contain a phosphoepitope shared by the animal SR family of splicing factors. In addition, we report the cloning and characterization of a cDNA encoding a higher-plant SR protein from Arabidopsis, SR1, which has striking sequence and structural homology to the human splicing factor SF2/ASF. Similar to SF2/ASF, the plant SR1 protein promotes splice site switching in mammalian nuclear extracts. A novel feature of the Arabidopsis SR protein is a C-terminal domain containing a high concentration of proline, serine, and lysine residues (PSK domain), a composition reminiscent of histones. This domain includes a putative phosphorylation site for the mitotic kinase cyclin/p34cdc2.

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Selected References

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