Abstract
A temperature-sensitive mutant of yeast, ts-187, which is apparently unable to initiate the synthesis of new polypeptide chains after a short incubation at the restrictive temperature, is described. The existence of this mutant demonstrates that in eucaryotic cells, as in procaryotic cells, there are processes unique to the initiation of polypeptide chains.
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- Clark B. F., Marcker K. A. N-formyl-methionyl-sigma-ribonucleic acid and chain initiation in protein biosynthesis. Polypeptide synthesis directed by a bacteriophage ribonucleic acid in a cell-free system. Nature. 1966 Jul 23;211(5047):378–380. doi: 10.1038/211378a0. [DOI] [PubMed] [Google Scholar]
- Hartwell L. H. Macromolecule synthesis in temperature-sensitive mutants of yeast. J Bacteriol. 1967 May;93(5):1662–1670. doi: 10.1128/jb.93.5.1662-1670.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hartwell L. H., McLaughlin C. S. Temperature-sensitive mutants of yeast exhibiting a rapid inhibition of protein synthesis. J Bacteriol. 1968 Nov;96(5):1664–1671. doi: 10.1128/jb.96.5.1664-1671.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hutchison H. T., Hartwell L. H. Macromolecule synthesis in yeast spheroplasts. J Bacteriol. 1967 Nov;94(5):1697–1705. doi: 10.1128/jb.94.5.1697-1705.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lucas-Lenard J., Lipmann F. Initiation of polyphenylalanine synthesis by N-acetylphenylalanyl-SRNA. Proc Natl Acad Sci U S A. 1967 Apr;57(4):1050–1057. doi: 10.1073/pnas.57.4.1050. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Revel M., Herzberg M., Becarevic A., Gros F. Role of protein factor in the functional binding of ribosomes to natural messenger RNA. J Mol Biol. 1968 Apr 14;33(1):231–249. doi: 10.1016/0022-2836(68)90291-x. [DOI] [PubMed] [Google Scholar]
- STENT G. S., BRENNER S. A genetic locus for the regulation of ribonucleic acid synthesis. Proc Natl Acad Sci U S A. 1961 Dec 15;47:2005–2014. doi: 10.1073/pnas.47.12.2005. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Salas M., Hille M. B., Last J. A., Wahba A. J., Ochoa S. Translation of the genetic message, ii. Effect of initiation factors on the binding of formyl-methionyl-trna to ribosomes. Proc Natl Acad Sci U S A. 1967 Feb;57(2):387–394. doi: 10.1073/pnas.57.2.387. [DOI] [PMC free article] [PubMed] [Google Scholar]