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. 1988 Nov;26(11):2371–2376. doi: 10.1128/jcm.26.11.2371-2376.1988

Optimal conditions for recovery of the human immunodeficiency virus from peripheral blood mononuclear cells.

B A Castro 1, C D Weiss 1, L D Wiviott 1, J A Levy 1
PMCID: PMC266895  PMID: 3266220

Abstract

Optimal conditions for demonstrating the presence of infectious human immunodeficiency virus in peripheral blood mononuclear cells (PMCs) from seropositive individuals involved cocultivation of infected cells with phytohemagglutinin-stimulated PMCs from seronegative donors in the presence of 2 micrograms of Polybrene per ml. The size of the culture vessel also influenced the results; smaller numbers of infected cells were detected under conditions of increased cell density. In addition, an increased normal donor/patient PMC ratio was helpful. The cocultivation approach permitted identification of human immunodeficiency virus in over 90% of seropositive individuals with different clinical conditions. Moreover, reconstruction experiments indicated that this method allows detection of one productively infected CD4+ cell in a population of over 10(6) PMCs.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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