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. 1987 Nov;61(11):3641–3644. doi: 10.1128/jvi.61.11.3641-3644.1987

Dissociation of NS5 from cell fractions containing West Nile virus-specific polymerase activity.

J B Grun 1, M A Brinton 1
PMCID: PMC255969  PMID: 2959795

Abstract

West Nile virus replication complexes were partially purified from cytoplasmic extracts of virus-infected cells by centrifugation through a 20% glycerol cushion. Numerous cell proteins, as well as the largest nonstructural protein, NS5, were separated from the replication complexes without significant loss of in vitro West Nile virus polymerase activity.

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Selected References

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