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. 1989 Nov;63(11):4919–4924. doi: 10.1128/jvi.63.11.4919-4924.1989

The NF-kappa B binding sites in the human immunodeficiency virus type 1 long terminal repeat are not required for virus infectivity.

J Leonard 1, C Parrott 1, A J Buckler-White 1, W Turner 1, E K Ross 1, M A Martin 1, A B Rabson 1
PMCID: PMC251138  PMID: 2795721

Abstract

Mutations were introduced into the regulatory sequences in the long terminal repeat of an infectious molecular clone of the human immunodeficiency virus. Viruses in which the NF-kappa B binding sites were deleted or ones in which one or two Sp1 binding sites were mutated still replicated efficiently in human T lymphocytes. A deletion of the two NF-kappa B sites plus the three Sp1 sites or a mutation of the tat-responsive region rendered the virus replication incompetent. Thus, the NF-kappa B sequences are not required for human immunodeficiency virus infectivity; however, a tat-responsive region is essential.

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Selected References

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