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. 1994 Sep;68(9):5982–5990. doi: 10.1128/jvi.68.9.5982-5990.1994

Sequence diversity of small, round-structured viruses in the Norwalk virus group.

J Wang 1, X Jiang 1, H P Madore 1, J Gray 1, U Desselberger 1, T Ando 1, Y Seto 1, I Oishi 1, J F Lew 1, K Y Green 1, et al.
PMCID: PMC237003  PMID: 8057474

Abstract

We have determined the nucleotide sequences of a highly conserved region of the RNA-dependent RNA polymerase of the prototype Snow Mountain agent (SMA) and of four other small, round-structured viruses (antigenically Norwalk virus [NV]-like or SMA-like) following reverse transcription-PCR amplification of viral RNA obtained from human stools. The stool samples were either from volunteers administered SMA or from sporadic cases and outbreaks of gastroenteritis that occurred in Japan and the United Kingdom between 1984 and 1992. The GLPSG and YGDD RNA polymerase motifs were in the proper locations in the sequences of the five SRSVs, but each sequence was distinct from the 8FIIa prototype NV sequence and from each other. Analysis of the sequences and reactivities in a new NV antigen enzyme-linked immunosorbent assay showed that the five viruses could be divided into two groups (serogroups) with NV and SMA, respectively, being the prototypes. The sequences of the capsid region and a nonstructural region (2C) were determined from one strain from each group. One virus (SRSV-KY-89/89/J), isolated in Japan and antigenically similar to the prototype NV (isolated 21 years earlier in Ohio), showed a remarkable level of sequence similarity to NV. KY-89 and the 8FIIa NV showed 87.2% nucleotide similarity over 2,516 continuous nucleotides amounting to 96 to 98.9% amino acid similarity in three distinct domains in two open reading frames. Between the prototype SMA and NV, the polymerase region showed 63% nucleotide and 59% amino acid similarity, respectively. Two other antigenically SMA-like isolates (SRSV-925/92/UK and SRSV-OTH-25/89/J), from the United Kingdom and Japan, showed 80% nucleotide and 88 to 92% amino acid similarity in the polymerase region to the prototype SMA isolated 16 and 13 years earlier in the United States. The capsid region of the antigenically SMA-like OTH-25 virus showed 53% nucleotide and 65% amino acid similarity to the prototype NV capsid region. Domains of sequence diversity and conversation were identified within the capsid protein of these two distinct prototype serotypes of virus. These results indicate that NV-like and SMA-like agents are still circulating, and sequence comparisons will be useful to identify and classify distinct viruses in the NV group.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appleton H. Small round viruses: classification and role in food-borne infections. Ciba Found Symp. 1987;128:108–125. doi: 10.1002/9780470513460.ch7. [DOI] [PubMed] [Google Scholar]
  2. Carter M. J., Milton I. D., Meanger J., Bennett M., Gaskell R. M., Turner P. C. The complete nucleotide sequence of a feline calicivirus. Virology. 1992 Sep;190(1):443–448. doi: 10.1016/0042-6822(92)91231-i. [DOI] [PubMed] [Google Scholar]
  3. Cubitt W. D., Blacklow N. R., Herrmann J. E., Nowak N. A., Nakata S., Chiba S. Antigenic relationships between human caliciviruses and Norwalk virus. J Infect Dis. 1987 Nov;156(5):806–814. doi: 10.1093/infdis/156.5.806. [DOI] [PubMed] [Google Scholar]
  4. Dolin R., Reichman R. C., Roessner K. D., Tralka T. S., Schooley R. T., Gary W., Morens D. Detection by immune electron microscopy of the Snow Mountain agent of acute viral gastroenteritis. J Infect Dis. 1982 Aug;146(2):184–189. doi: 10.1093/infdis/146.2.184. [DOI] [PubMed] [Google Scholar]
  5. Gary G. W., Jr, Kaplan J. E., Stine S. E., Anderson L. J. Detection of Norwalk virus antibodies and antigen with a biotin-avidin immunoassay. J Clin Microbiol. 1985 Aug;22(2):274–278. doi: 10.1128/jcm.22.2.274-278.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Graham D. Y., Jiang X., Tanaka T., Opekun A. R., Madore H. P., Estes M. K. Norwalk virus infection of volunteers: new insights based on improved assays. J Infect Dis. 1994 Jul;170(1):34–43. doi: 10.1093/infdis/170.1.34. [DOI] [PubMed] [Google Scholar]
  7. Herrmann J. E., Nowak N. A., Blacklow N. R. Detection of Norwalk virus in stools by enzyme immunoassay. J Med Virol. 1985 Oct;17(2):127–133. doi: 10.1002/jmv.1890170205. [DOI] [PubMed] [Google Scholar]
  8. Jiang X., Wang J., Graham D. Y., Estes M. K. Detection of Norwalk virus in stool by polymerase chain reaction. J Clin Microbiol. 1992 Oct;30(10):2529–2534. doi: 10.1128/jcm.30.10.2529-2534.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jiang X., Wang M., Graham D. Y., Estes M. K. Expression, self-assembly, and antigenicity of the Norwalk virus capsid protein. J Virol. 1992 Nov;66(11):6527–6532. doi: 10.1128/jvi.66.11.6527-6532.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jiang X., Wang M., Wang K., Estes M. K. Sequence and genomic organization of Norwalk virus. Virology. 1993 Jul;195(1):51–61. doi: 10.1006/viro.1993.1345. [DOI] [PubMed] [Google Scholar]
  11. Kapikian A. Z., Wyatt R. G., Dolin R., Thornhill T. S., Kalica A. R., Chanock R. M. Visualization by immune electron microscopy of a 27-nm particle associated with acute infectious nonbacterial gastroenteritis. J Virol. 1972 Nov;10(5):1075–1081. doi: 10.1128/jvi.10.5.1075-1081.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lambden P. R., Caul E. O., Ashley C. R., Clarke I. N. Sequence and genome organization of a human small round-structured (Norwalk-like) virus. Science. 1993 Jan 22;259(5094):516–519. doi: 10.1126/science.8380940. [DOI] [PubMed] [Google Scholar]
  13. Lew J. F., Petric M., Kapikian A. Z., Jiang X., Estes M. K., Green K. Y. Identification of minireovirus as a Norwalk-like virus in pediatric patients with gastroenteritis. J Virol. 1994 May;68(5):3391–3396. doi: 10.1128/jvi.68.5.3391-3396.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lewis D. C. Three serotypes of Norwalk-like virus demonstrated by solid-phase immune electron microscopy. J Med Virol. 1990 Jan;30(1):77–81. doi: 10.1002/jmv.1890300117. [DOI] [PubMed] [Google Scholar]
  15. Lewis D. Norwalk agent and other small-round structured viruses in the U.K. J Infect. 1991 Sep;23(2):220–222. doi: 10.1016/0163-4453(91)92567-o. [DOI] [PubMed] [Google Scholar]
  16. Madore H. P., Treanor J. J., Pray K. A., Dolin R. Enzyme-linked immunosorbent assays for Snow Mountain and Norwalk agents of viral gastroenteritis. J Clin Microbiol. 1986 Sep;24(3):456–459. doi: 10.1128/jcm.24.3.456-459.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Meyers G., Wirblich C., Thiel H. J. Rabbit hemorrhagic disease virus--molecular cloning and nucleotide sequencing of a calicivirus genome. Virology. 1991 Oct;184(2):664–676. doi: 10.1016/0042-6822(91)90436-f. [DOI] [PubMed] [Google Scholar]
  18. Moe C. L., Gentsch J., Ando T., Grohmann G., Monroe S. S., Jiang X., Wang J., Estes M. K., Seto Y., Humphrey C. Application of PCR to detect Norwalk virus in fecal specimens from outbreaks of gastroenteritis. J Clin Microbiol. 1994 Mar;32(3):642–648. doi: 10.1128/jcm.32.3.642-648.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Morens D. M., Zweighaft R. M., Vernon T. M., Gary G. W., Eslien J. J., Wood B. T., Holman R. C., Dolin R. A waterborne outbreak of gastroenteritis with secondary person-to-person spread. Association with a viral agent. Lancet. 1979 May 5;1(8123):964–966. doi: 10.1016/s0140-6736(79)91734-3. [DOI] [PubMed] [Google Scholar]
  20. Neill J. D. Nucleotide sequence of the capsid protein gene of two serotypes of San Miguel sea lion virus: identification of conserved and non-conserved amino acid sequences among calicivirus capsid proteins. Virus Res. 1992 Jul;24(2):211–222. doi: 10.1016/0168-1702(92)90008-w. [DOI] [PubMed] [Google Scholar]
  21. Neill J. D., Reardon I. M., Heinrikson R. L. Nucleotide sequence and expression of the capsid protein gene of feline calicivirus. J Virol. 1991 Oct;65(10):5440–5447. doi: 10.1128/jvi.65.10.5440-5447.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Numata K., Nakata S., Jiang X., Estes M. K., Chiba S. Epidemiological study of Norwalk virus infections in Japan and Southeast Asia by enzyme-linked immunosorbent assays with Norwalk virus capsid protein produced by the baculovirus expression system. J Clin Microbiol. 1994 Jan;32(1):121–126. doi: 10.1128/jcm.32.1.121-126.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Oishi I., Yamazaki K., Kimoto T., Minekawa Y. Demonstration of low molecular weight polypeptides associated with small, round-structured viruses by western immunoblot analysis. Microbiol Immunol. 1992;36(10):1105–1112. doi: 10.1111/j.1348-0421.1992.tb02114.x. [DOI] [PubMed] [Google Scholar]
  24. Okada S., Sekine S., Ando T., Hayashi Y., Murao M., Yabuuchi K., Miki T., Ohashi M. Antigenic characterization of small, round-structured viruses by immune electron microscopy. J Clin Microbiol. 1990 Jun;28(6):1244–1248. doi: 10.1128/jcm.28.6.1244-1248.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Thornhill T. S., Wyatt R. G., Kalica A. R., Dolin R., Chanock R. M., Kapikian A. Z. Detection by immune electron microscopy of 26- to 27-nm viruslike particles associated with two family outbreaks of gastroenteritis. J Infect Dis. 1977 Jan;135(1):20–27. doi: 10.1093/infdis/135.1.20. [DOI] [PubMed] [Google Scholar]
  26. Tohya Y., Taniguchi Y., Takahashi E., Utagawa E., Takeda N., Miyamura K., Yamazaki S., Mikami T. Sequence analysis of the 3'-end of feline calicivirus genome. Virology. 1991 Aug;183(2):810–814. doi: 10.1016/0042-6822(91)91016-A. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Treanor J. J., Jiang X., Madore H. P., Estes M. K. Subclass-specific serum antibody responses to recombinant Norwalk virus capsid antigen (rNV) in adults infected with Norwalk, Snow Mountain, or Hawaii virus. J Clin Microbiol. 1993 Jun;31(6):1630–1634. doi: 10.1128/jcm.31.6.1630-1634.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Wyatt R. G., Dolin R., Blacklow N. R., DuPont H. L., Buscho R. F., Thornhill T. S., Kapikian A. Z., Chanock R. M. Comparison of three agents of acute infectious nonbacterial gastroenteritis by cross-challenge in volunteers. J Infect Dis. 1974 Jun;129(6):709–714. doi: 10.1093/infdis/129.6.709. [DOI] [PubMed] [Google Scholar]
  29. Xi J. N., Graham D. Y., Wang K. N., Estes M. K. Norwalk virus genome cloning and characterization. Science. 1990 Dec 14;250(4987):1580–1583. doi: 10.1126/science.2177224. [DOI] [PubMed] [Google Scholar]

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