Abstract
A method of in situ perfusion of rat liver via the portal vein is described, by which osmiumtetroxide fixative can be introduced rapidly and uniformly to all parts of the tissue. Previous perfusion with balanced physiological saline solution, under the conditions described, of itself causes minimal change in the liver cell and liver architecture. Perfusion with a chelating agent causes no further detectable alteration within the liver cell but results in separation of the cells, even to the extent of producing a free cell suspension. The separation of cells is not accompanied by any recognizable damage to the plasma membrane but there is a striking tendency to pseudopod formation on the newly exposed surface. These findings provide direct evidence to support the classical concept of the importance of calcium to the adhesiveness of the plasma membrane in the tissues of the adult rat.
Full Text
The Full Text of this article is available as a PDF (1.0 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ANDERSON N. G. The mass isolation of whole cells from rat liver. Science. 1953 Jun 5;117(3049):627–628. doi: 10.1126/science.117.3049.627. [DOI] [PubMed] [Google Scholar]
- COMAN D. R. Cellular adhesiveness in relation to the invasiveness of cancer; electron microscopy of liver perfused with a chelating agent. Cancer Res. 1954 Aug;14(7):519–521. [PubMed] [Google Scholar]
- EASTY G. C., MUTOLO V. The nature of the intercellular material of adult mammalian tissues. Exp Cell Res. 1960 Nov;21:374–385. doi: 10.1016/0014-4827(60)90269-x. [DOI] [PubMed] [Google Scholar]
- FAWCETT D. W. Observations on the cytology and electron microscopy of hepatic cells. J Natl Cancer Inst. 1955 Apr;15(5 Suppl):1475–1503. [PubMed] [Google Scholar]
- KALANT H., YOUNG F. G. Metabolic behaviour of isolated liver and kidney cells. Nature. 1957 Apr 20;179(4564):816–817. doi: 10.1038/179816a0. [DOI] [PubMed] [Google Scholar]
- LATA G. F., REINERTSON J. Cholesterol synthesis by isolated rat liver cells. Nature. 1957 Jan 5;179(4549):47–48. doi: 10.1038/179047a0. [DOI] [PubMed] [Google Scholar]
- LAWS J. O., STRICKLAND L. H. Metabolism of isolated liver cells. Nature. 1956 Aug 11;178(4528):309–310. doi: 10.1038/178309a0. [DOI] [PubMed] [Google Scholar]
- PALADE G. E., PORTER K. R. Studies on the endoplasmic reticulum. I. Its identification in cells in situ. J Exp Med. 1954 Dec 1;100(6):641–656. doi: 10.1084/jem.100.6.641. [DOI] [PMC free article] [PubMed] [Google Scholar]