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. 1967 May 1;125(5):755–765. doi: 10.1084/jem.125.5.755

IMMUNOFLUORESCENT STUDIES OF THE POTENTIATION OF AN ADENOVIRUS-ASSOCIATED VIRUS BY ADENOVIRUS 7

Neil R Blacklow 1, M David Hoggan 1, Wallace P Rowe 1
PMCID: PMC2138211  PMID: 4225814

Abstract

A quantitative immunofluorescent procedure for detection of viral antigen was used to study the potentiation of AAV-1 by Ad.7. AAV viral antigen formed only when the cells were also infected with adenovirus, and only in cell culture systems in which the adenovirus infection proceeded to completion. Ad. 7 infection of AGMK. cell cultures did not potentiate AAV unless the Ad. 7 infection was itself potentiated by SV40. Dose-response studies indicated that a single AAV particle and a single infectious Ad. 7 particle sufficed to initiate AAV antigen synthesis. Sequential inoculation studies showed that AAV antigen formed simultaneously with Ad. 7 viral antigen when the AAV was inoculated any time between 15 hr before to 10 hr after the Ad. 7, both antigens appearing about 15 hr after inoculation of Ad. 7. The AAV-1 antigen formation had a minimum latent period of 5 hr, as seen with Ad. 7 preinfection of 10 hr or more. When UV-irradiated Ad. 7 was used as helper, the AAV antigen still appeared simultaneously with the Ad. 7 viral antigen, even though the latter was delayed by 23 hr compared to nonirradiated virus. When the early replicative events of both viruses were allowed to proceed in FUDR-inhibited cells, and then the FUDR inhibition was reversed, AAV antigen formed within 2 hr, which was 3 hr before the Ad. 7 viral antigen appeared. It was inferred that the event in the adenovirus cycle that renders a cell competent to synthesize AAV occurs after the 10th hr and may be temporally associated with replication of the adenovirus DNA.

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Selected References

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  1. ATCHISON R. W., CASTO B. C., HAMMON W. M. ADENOVIRUS-ASSOCIATED DEFECTIVE VIRUS PARTICLES. Science. 1965 Aug 13;149(3685):754–756. doi: 10.1126/science.149.3685.754. [DOI] [PubMed] [Google Scholar]
  2. FLANAGAN J. F., GINSBERG H. S. Synthesis of virus-specific polymers in adenovirus-infected cells; effect of 5-fluorodeoxyuridine. J Exp Med. 1962 Aug 1;116:141–157. doi: 10.1084/jem.116.2.141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. GINSBERG H. S., DIXON M. K. Nucleuc acid synthesis in types 4 and 5 adenovirus-infected HeLa cells. J Exp Med. 1961 Feb 1;113:283–299. doi: 10.1084/jem.113.2.283. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. GREEN M., DAESCH G. E. Biochemical studies on adenovirus multiplication. II. Kinetics of nucleic acid and protein synthesis in suspension cultures. Virology. 1961 Feb;13:169–176. doi: 10.1016/0042-6822(61)90051-4. [DOI] [PubMed] [Google Scholar]
  5. GREEN M. Studies on the biosynthesis of viral DNA. Cold Spring Harb Symp Quant Biol. 1962;27:219–235. doi: 10.1101/sqb.1962.027.001.022. [DOI] [PubMed] [Google Scholar]
  6. Gilead Z., Ginsberg H. S. Characterization of a Tumorlike Antigen in Type 12 and Type 18 Adenovirus-Infected Cells. J Bacteriol. 1965 Jul;90(1):120–125. doi: 10.1128/jb.90.1.120-125.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HIATT C. W. KINETICS OF THE INACTIVATION OF VIRUSES. Bacteriol Rev. 1964 Jun;28:150–163. doi: 10.1128/br.28.2.150-163.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hoggan M. D., Blacklow N. R., Rowe W. P. Studies of small DNA viruses found in various adenovirus preparations: physical, biological, and immunological characteristics. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1467–1474. doi: 10.1073/pnas.55.6.1467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. POPE J. H., ROWE W. P. DETECTION OF SPECIFIC ANTIGEN IN SV40-TRANSFORMED CELLS BY IMMUNOFLUORESCENCE. J Exp Med. 1964 Aug 1;120:121–128. doi: 10.1084/jem.120.2.121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. RABSON A. S., O'CONOR G. T., BEREZESKY I. K., PAUL F. J. ENHANCEMENT OF ADENOVIRUS GROWTH IN AFRICAN GREEN MONKEY KIDNEY CELL CULTURES BY SV40. Proc Soc Exp Biol Med. 1964 May;116:187–190. doi: 10.3181/00379727-116-29197. [DOI] [PubMed] [Google Scholar]
  11. Reich P. R., Baum S. G., Rose J. A., Rowe W. P., Weissman S. M. Nucleic acid homology studies of adenovirus type 7-SV40 interactions. Proc Natl Acad Sci U S A. 1966 Feb;55(2):336–341. doi: 10.1073/pnas.55.2.336. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rowe W. P., Baum S. G., Pugh E., Hoggan M. D. Studies of adenovirus SV40 hybrid viruses. I. Assay system and further evidence for hybridization. J Exp Med. 1965 Nov 1;122(5):943–954. doi: 10.1084/jem.122.5.943. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. SMITH C. W., MARSHALL J. D., Jr, EVELAND W. C. Use of contrasting fluorescent dye as counterstain in fixed tissue preparations. Proc Soc Exp Biol Med. 1959 Oct;102:179–181. doi: 10.3181/00379727-102-25182. [DOI] [PubMed] [Google Scholar]

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