Abstract
The phagocytosis and intracellular destruction of bacteria by rabbit polymorphonuclear leucocytes has been studied in vitro under defined conditions. The efficient and continuing ingestion of bacteria was dependent upon (a) opsonic factors present in fresh rabbit serum as well as upon, (b) the availability of an adequate supply of glucose in the medium. The effects of selected enzymatic inhibitors on the metabolic and functional activities of the leucocytes was investigated. Cyanide which inhibited oxygen consumption had no effect on the ingestion or inactivation of bacteria, Iodoacetate and arsenite which blocked glycolysis produced a marked inhibition in particle ingestion. 2,4-Dinitrophenol which stimulated both oxygen consumption and glycolysis, depressed phagocytosis after a 1 hour latent period. It was concluded that phagocytosis was an energy-requiring process in which glycolysis served as the most important source of energy. Leucocytes which were ingesting heat-killed bacteria exhibited increases in oxygen consumption, glucose utilization, and lactic acid synthesis. The effect of particle ingestion on glycogen metabolism was characterized by an initial period of glycogenolysis followed by an enhanced rate of glycogen synthesis. Leucocytes which had previously ingested heat-killed bacteria also demonstrated increased rates of phagocytosis.
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Selected References
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- BAZIN S., AVICE C. Le métabolisme glycogénique des polynucléaires au cours de la phagocytose in vitro. C R Seances Soc Biol Fil. 1953 Jun;147(11-12):1025–1027. [PubMed] [Google Scholar]
- BERRY L. J., SPIES T. D. Phagocytosis. Medicine (Baltimore) 1949 Sep;28(3):239–300. doi: 10.1097/00005792-194909000-00001. [DOI] [PubMed] [Google Scholar]
- COHN Z. A., MORSE S. I. Interactions between rabbit polymorphonuclear leucocytes and staphylococci. J Exp Med. 1959 Sep 1;110:419–443. doi: 10.1084/jem.110.3.419. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FISHER T. N., GINSBERG H. S. The reaction of influenza viruses with guinea pig polymorphonuclear leucocytes. II. The reduction of white blood cell glycolysis by influenza viruses and receptor-destroying enzyme (RDE). Virology. 1956 Oct;2(5):637–655. doi: 10.1016/0042-6822(56)90044-7. [DOI] [PubMed] [Google Scholar]
- KIPNIS D. M., CORI C. F. Studies of tissue permeability. V. The penetration and phosphorylation of 2-deoxyglucose in the rat diaphragm. J Biol Chem. 1959 Jan;234(1):171–177. [PubMed] [Google Scholar]
- SBARRA A. J., KARNOVSKY M. L. The biochemical basis of phagocytosis. I. Metabolic changes during the ingestion of particles by polymorphonuclear leukocytes. J Biol Chem. 1959 Jun;234(6):1355–1362. [PubMed] [Google Scholar]
- STAHELIN H., KARNOVSKY M. L., FARNHAM A. E., SUTER E. Studies on the interaction between phagocytes and tubercle bacilli. III. Some metabolic effects in guinea pigs associated with infection with tubercle bacilli. J Exp Med. 1957 Mar 1;105(3):265–277. doi: 10.1084/jem.105.3.265. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STAHELIN H., SUTER E., KARNOVSKY M. L. Studies on the interaction between phagocytes and tubercle bacilli. I. Observations on the metabolism of guinea pig leucocytes and the influence of phagocytosis. J Exp Med. 1956 Jul 1;104(1):121–136. doi: 10.1084/jem.104.1.121. [DOI] [PMC free article] [PubMed] [Google Scholar]